Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	580,020	0	G	A	55.9%	7.3 / 36.5	34	R47R (AGG→AGA)	ybcW	uncharacterized protein YbcW
Reads supporting (aligned to +/- strand):  ref base G (9/6);  new base A (9/10);  total (18/16)
Fisher's exact test for biased strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTT  >  NC_000913/579776‑580267                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        aaCCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAAtt                                                                                                                                                                                                                                                   <  1:214793/251‑1 (MQ=255)         gATGTATGTAAGTCAAACGTGCTCAAATCTTAAGACTGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATTGAGCTACATAATGTATACAGAACAATCTGCTGATGATCCCTCCTTGGATCTGATTCGTGTAAAAAATATTCTTAATATCACCATTTCGATGAGTTACCCTGAAGTTGTAAGTGCATTTATAGAACATAAGGTGTCTCTAGAAGCATTCAGAGCAAtttagacatc                                                                                                                                                                                                                                           <  2:132974/251‑9 (MQ=255)           tgtatgtaAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGt                                                                                                                                                                                                                                         >  1:35509/1‑251 (MQ=255)                                 aaaCCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATATGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAACATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGAGAAGCACGATAATAatat                                                                                                                                                                                                                   >  1:31279/1‑251 (MQ=255)                                             aGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGAAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATCATAATATGAAGGATTATTc                                                                                                                                                                                                       >  1:198754/1‑251 (MQ=255)                                                  gATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCtgg                                                                                                                                                                                                  <  2:156258/251‑1 (MQ=255)                                                                  ttAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCAcc                                                                                                                                                                                  >  2:74670/1‑251 (MQ=255)                                                                          tAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGc                                                                                                                                                                          >  1:163444/1‑251 (MQ=255)                                                                                   agCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGAATCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGCAATTGCATGAATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTAGGTGAAGCACGATAATAATATGCAGGCTTATTCCCTGGTGGTTGACTGCTCACCATAACTGATAATCATTc                                                                                                                                                                 >  2:279684/1‑251 (MQ=255)                                                                                      aaGCGCATGGAGCGACAAAATGAATAAAGAACAATCTCCAAATAATCCCTCCCTGGAACTGATTCCTGCAACAAATATGCTTAATAGCACCATTTATATGAGTTACCCTGATGTTGCAATTGCATGTATAGACCATAAGGTGTCTCTGGAAGAATTCAGAGAAATTGAGGCAGCGTTGGTGAAGCACGAAACTAATATGAAGGATTATTCCCTGGTGGTTGACTGATAACCATAACTGCTAATCATTCaa                                                                                                                                                               >  1:202260/1‑250 (MQ=255)                                                                                                           gAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACagag                                                                                                                                          <  2:172555/250‑1 (MQ=255)                                                                                                                      aaTCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAgg                                                                                                                                                                                                              >  1:65889/1‑171 (MQ=255)                                                                                                                      aaTCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAgg                                                                                                                                                                                                              <  2:65889/171‑1 (MQ=255)                                                                                                                      aaTCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGt                                                                                                                              >  2:76380/1‑251 (MQ=255)                                                                                                                                 gatCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTctgtcactgtc                                                                                                                   <  1:76380/251‑1 (MQ=255)                                                                                                                                          gTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACt                                                                                                                                                                            >  1:354343/1‑185 (MQ=255)                                                                                                                                          gTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCGGGTGGTTGACTGATCACCATAACt                                                                                                                                                                            <  2:354343/185‑1 (MQ=255)                                                                                                                                            ggATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGt                                                                                                        <  1:74670/251‑1 (MQ=255)                                                                                                                                             gATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGt                                                                                                        >  2:199048/1‑250 (MQ=255)                                                                                                                                                                  atGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACAATAACTGCTAATCATGCAAACTACTTAACCTGTGACAGAGCCAACACGCCGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGc                                                                                  >  2:80881/1‑251 (MQ=255)                                                                                                                                                                     cTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAg                                                                                                                 <  2:285109/217‑1 (MQ=255)                                                                                                                                                                     cTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAg                                                                                                                 >  1:285109/1‑217 (MQ=255)                                                                                                                                                                       tAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGc                                                                             >  1:120734/1‑251 (MQ=255)                                                                                                                                                                       tAATAGCACCATTTATATGAGTTACCCTGCTGTTGTAATTGCATGTCTAGCACATAAGGTGTCTCTGGCAGCATTCAGGGCCATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCacac                                                                                                                                                             >  2:158820/1‑168 (MQ=255)                                                                                                                                                                       tAATAGCACCATGTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAac                                                                                                                                                             <  1:158820/171‑1 (MQ=255)                                                                                                                                                                         aTAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGccc                                                                           >  2:98623/1‑251 (MQ=255)                                                                                                                                                                                    tCTATGAGTTACCCTGATGTTGTAATTGCATGTATCGACCATCAGGTGTCTCTCGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATCATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCATACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCATCGTAATTa                                                                  >  1:324346/1‑249 (MQ=255)                                                                                                                                                                                    tCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTaa                                                                 <  1:87137/250‑1 (MQ=255)                                                                                                                                                                                          aGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTTGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGTTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAAtt                                                          <  1:199048/251‑1 (MQ=255)                                                                                                                                                                                                    aTGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGCAGCATTCAGGGCAATTGCGGCAGCGTTGGCGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTAAGGAAAGTGGTAAAACTGCAACTCAATCACTGCAATGCCCTCGTACTTAAGTGAATTTACAATATCg                                                >  1:308566/1‑251 (MQ=255)                                                                                                                                                                                                      gttgtAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAAAATCGTc                                              <  1:99351/251‑1 (MQ=255)                                                                                                                                                                                                        tgtAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTTGTGGTTGACTGATCACCATAACTGCTAAGCAGTCAAACTATGGAGTCTGTGACAGAGCCAACATGTAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACCGCTTTGTCCTCGTAATTAAGTGAATTTACAATATCGTcc                                             <  1:315305/250‑1 (MQ=255)                                                                                                                                                                                                                                   gtCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGTATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGATCCAACACGCAGTCTGTCACTGTCAGTAAAGTGGTAAAACTGCAACTCAATTACTACAATGCCCTCGTAATTAAGTTAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTc                 <  1:298755/251‑1 (MQ=255)                                                                                                                                                                                                                                                  cAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACttt  <  1:72573/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        AACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTT  >  NC_000913/579776‑580267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑