Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913579,3850AG63.2% 7.6 / 16.8 19N70N (AAT→AAC) ybcVDUF1398 domain‑containing protein YbcV
Reads supporting (aligned to +/- strand):  ref base A (3/4);  new base G (3/9);  total (6/13)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.75e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCG  >  NC_000913/579173‑579626
                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 
gACGTTATGATTTAGCGTTGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTcc                                                                                                                                                                                                             <  2:81114/251‑1 (MQ=255)
             aGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAacac                                                                                                                                                                                                 <  1:69072/250‑1 (MQ=255)
                             tgtAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAaca                                                                                                                                                                                                  <  2:69241/233‑1 (MQ=255)
                             tgtAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAaca                                                                                                                                                                                                  >  1:69241/1‑233 (MQ=255)
                                                   gTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATGTTATCtgt                                                                                                                                                          <  2:133495/251‑1 (MQ=255)
                                                               gAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTTGATAGt                                                                                                                                               <  1:296494/250‑1 (MQ=255)
                                                                         aGGTATAATAATATCTTTTATGTTCGTTGATATTTGTAATCCACCGGCAAACGCCTGCTTTAGCAAGATTTTCCCTGGAGTGCTGAATTGTTATTTCTCTTGATTTCAACCTATCATAGGACGTTGCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTAGCTGTGTCGAGATAGTAAATATAAtgt                                                                                                                                    <  2:240735/251‑1 (MQ=255)
                                                                                         tttATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGAc                                                                                                                       <  1:100035/248‑1 (MQ=255)
                                                                                         tttATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGAAAGATTTTCCCTGTATTGCTGCAATGTGATTTCTCTTGATTTCAACCTCTCATAGTACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACCCGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATCGTAAATATAATGTGAGACGTTGTGACGtt                                                                                                                    >  1:299613/1‑251 (MQ=255)
                                                                                          ttATGTTCATGGATATTTGTAACCCATCAGAAAACTCCTGCTTTAGCAAGATTTGTCCTGTATTGCTGAAATGTGATTTCTCTTGATGTCAACCTCTACTAGGACGTTTCTAGAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTAATCGTATTCTAACCCGATGTGAGTAGTATCAGAGGTTAGATAGTAAACATCATGTGAGACGTtgggccgtt                                                                                                                    <  2:82388/250‑8 (MQ=255)
                                                                                                                                     tAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCttt                                                                               <  2:82510/244‑1 (MQ=255)
                                                                                                                                     tAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCttt                                                                               >  1:82510/1‑244 (MQ=255)
                                                                                                                                            aTTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCTTAGGACGTTTCTATAAGATGCGCATTTCTTGCGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGCTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATACTGTGAGACATTGTGACGTTTTAGTTCAGAATAAACCAATTCACAGTTTAAATCTTTTCGCACTTGATCg                                                                  >  1:32879/1‑250 (MQ=255)
                                                                                                                                                     gtagtGCTGAAATGTGATTTAGCTGGATTTCAACCTATCATAGGACGTGTCTATAAGATGCGTATTGCTTGAGAATTTAACATTTACATCCTTTTTAAGTCCTTTGATTATCACGGTGTTATCGTGTTCTAATACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTGCAGAATAAAACCATTCACAGTGTAAATCTGTGCTCACTTGATCGGATATTTc                                                          <  2:313466/245‑1 (MQ=255)
                                                                                                                                                             gAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATg                                                >  1:306381/1‑251 (MQ=255)
                                                                                                                                                                    gATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAAcc                                          <  1:72348/250‑1 (MQ=255)
                                                                                                                                                                        tctcTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGc                                     >  1:156258/1‑251 (MQ=255)
                                                                                                                                                                              gATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAAGAATTCACATTTTAAGTCTTTTCGGTCTTTATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTgg                               <  1:25384/251‑1 (MQ=255)
                                                                                                                                                                                                           aaGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGcgcg  <  1:199749/251‑1 (MQ=255)
                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 
GACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCG  >  NC_000913/579173‑579626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑