Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	579,285	0	T	C	58.8%	9.3 / 10.5	17	I104V (ATT→GTT)	ybcV	DUF1398 domain‑containing protein YbcV
Reads supporting (aligned to +/- strand):  ref base T (3/4);  new base C (3/7);  total (6/11)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAAACGATGCACACAGGGTTTAGCGCGTACATGTATTGTATTATGCCAACACCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTT  >  NC_000913/579039‑579512                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                    aaCTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGCATAATTAAAAACGATGCACACAGGGATTAGCGCGTACATGTATTGTATTATGCCAACACCCCGGTGCTGACACGGAAGAAACCGAACGTTATGATTTAGCGTGGAAAGCTTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTCTTGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCAt                                                                                                                                                                                                                                 >  2:9660/1‑251 (MQ=255)     aTGTCCACAGCCCTGCCGGGGAACTTCTCTGCGGGAGTCTCCGGGTATAATTCAAA‑CGATGCACACCGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCACCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTCAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGa                                                                                                                                                                                                                             >  2:136043/1‑250 (MQ=25)              gCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAAACGATGCACACAGGGTTTAGCGCGTACATGTATTGTATTATGCCAACACCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCGGAAAATTCCTg                                                                                                                                                                                                                    <  1:13121/251‑1 (MQ=255)                   gACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAA‑CGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTa                                                                                                                                                                                                               <  2:82665/250‑1 (MQ=37)                     cGGGGAACTTCTCTGCGGGAGTGGCCGGGAATAATTAAAAACGATGCACACCGGGTTTAGCGCGTACATGTATTGTATTATGCCAACACCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTACATAGTAATGAATTATCAAAGGCATAGTACTTTCTTTTATGTTCGTGGATATTTGTAATCCATCGGAACACTCCTTCTTTAGc                                                                                                                                                                                                             >  2:38675/1‑251 (MQ=255)                                                          aaaaCGATGCACACAGGGTTTAGCGCGTACATGTATTGTATTATGCCAGCACCCCGGTGCTGACACGGAAGAAACCTGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCGTTTATGGGCGTGGATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTGTCCCTGTATTGCTGATATGTGATTTCTCtt                                                                                                                                                                        <  2:247034/251‑1 (MQ=37)                                                                                             ttgtattATGCCAACACCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATg                                                                                                                                     >  1:250519/1‑251 (MQ=255)                                                                                                                                       gACGTTATGATTTAGCGTTGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTcc                                                                                           <  2:81114/251‑1 (MQ=255)                                                                                                                                                    aGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAacac                                                                               <  1:69072/250‑1 (MQ=255)                                                                                                                                                                    tgtAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAaca                                                                                >  1:69241/1‑233 (MQ=255)                                                                                                                                                                    tgtAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAaca                                                                                <  2:69241/233‑1 (MQ=255)                                                                                                                                                                                          gTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATGTTATCtgt                                        <  2:133495/251‑1 (MQ=255)                                                                                                                                                                                                      gAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTTGATAGt                             <  1:296494/250‑1 (MQ=255)                                                                                                                                                                                                                aGGTATAATAATATCTTTTATGTTCGTTGATATTTGTAATCCACCGGCAAACGCCTGCTTTAGCAAGATTTTCCCTGGAGTGCTGAATTGTTATTTCTCTTGATTTCAACCTATCATAGGACGTTGCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTAGCTGTGTCGAGATAGTAAATATAAtgt                  <  2:240735/251‑1 (MQ=255)                                                                                                                                                                                                                                tttATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGAc     <  1:100035/248‑1 (MQ=255)                                                                                                                                                                                                                                tttATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGAAAGATTTTCCCTGTATTGCTGCAATGTGATTTCTCTTGATTTCAACCTCTCATAGTACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACCCGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATCGTAAATATAATGTGAGACGTTGTGACGtt  >  1:299613/1‑251 (MQ=255)                                                                                                                                                                                                                                 ttATGTTCATGGATATTTGTAACCCATCAGAAAACTCCTGCTTTAGCAAGATTTGTCCTGTATTGCTGAAATGTGATTTCTCTTGATGTCAACCTCTACTAGGACGTTTCTAGAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTAATCGTATTCTAACCCGATGTGAGTAGTATCAGAGGTTAGATAGTAAACATCATGTGAGACGTtgggccgtt  <  2:82388/250‑8 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                    AACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAAACGATGCACACAGGGTTTAGCGCGTACATGTATTGTATTATGCCAACACCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTT  >  NC_000913/579039‑579512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑