Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,252,952	IS2 (–) +5 bp	coding (42‑46/357 nt)	yhaI →	putative inner membrane protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3186096 =	NA (NA)	33 (0.970)	31/486	0.2	100%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	3252952 =	0 (0.000)					coding (42/357 nt)	yhaI	putative inner membrane protein
*	?	NC_000913	= 3187426	NA (NA)	27 (0.790)	26/486	0.4	100%	noncoding (1331/1331 nt)	IS2	repeat region
	?	NC_000913	= 3252956	0 (0.000)					coding (46/357 nt)	yhaI	putative inner membrane protein

CAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGCAAT  >  NC_000913/3185848‑3186343                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                        caacaaTAACAACAGCTATCAGAAACTGAATTTCAAAGATCGTATGGAATCCCCAAGCAAAGATGCCACCCAGGGCAACGTTTACGCTCCGGCTGACTACAATATTGCTTACGAGTAATCCCATTTTGCCGGGGTGGCCCGGCCACATCGTTAACGGATTAATGATCAGTGGATCAGCTGTATAAAAAATTAAAATTAACAACCATAAGCGAATTGATTAACAATATATATTGTTCATTATCCGttatt                                                                                                                                                                                                                                                         >  1:237514/1‑249 (MQ=255)    caaTACCAACAGCTATCAGGAACTGAATTTCAAAGCACGTATGGAATCCCCAAGAACAGATGCCACCCAGGGCAACGTTTACGCTCAGGCTGAATACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACt                                                                                                                                                                                                                                                    >  1:166648/1‑251 (MQ=255)                                 tCAAATCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAAGTAACCACAATAAGCGAATTTATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCGGACAACCAata                                                                                                                                                                                                                        <  2:333734/250‑1 (MQ=255)                                  cAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATc                                                                                                                                                                                                                      >  1:113555/1‑251 (MQ=255)                                                                           gCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGAGAGTCTTAATACTAGTTTTTAGACTAg                                                                                                                                                                             <  2:162887/251‑1 (MQ=255)                                                                                         cAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCTGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCTGTTATTAGACTGGCCTCCTGAATCTCCAGACAACCAATATCTTGTAAATAAGTGATAGTTTTAATACTAGTTTTTAGACTAGTCATTGGAGAACa                                                                                                                                                                <  2:177543/250‑1 (MQ=38)                                                                                            gCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAgatg                                                                                                                                                            >  2:150993/1‑251 (MQ=255)                                                                                              tGACTACAAGATTGCTTACGAGTAATCGCATTTGGCAGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTCTCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATACGTGATAGTCTTAATACTAGTTTTTAGACTAGCAATTGGAGAACAgatgat                                                                                                                                                          >  2:241737/1‑251 (MQ=255)                                                                                               gACTACAAGATTGCATACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTTTTCATTATCCGTTATTAGACTGGCCACCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAgatga                                                                                                                                                           <  1:348722/249‑1 (MQ=255)                                                                                                                                    ggTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACca                                                                                                                    <  1:150993/251‑1 (MQ=255)                                                                                                                                        cGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGTCCCCCTGAATCTCCAGACAACCAATATCACTGAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACcacac                                                                                                                 <  2:351057/250‑1 (MQ=255)                                                                                                                                                        tAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAAtt                                                                                                >  1:166584/1‑251 (MQ=255)                                                                                                                                                            ggATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGtt                                                                                                                                                                                       >  1:49527/1‑160 (MQ=255)                                                                                                                                                            ggATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATGAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGtt                                                                                                                                                                                       <  2:49527/160‑1 (MQ=255)                                                                                                                                                              aTTAATGATAAGTGGATCAGATGTAGAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCTCTTAAATAAGTGATAGTCTTAATACTAGTTTTTATACTAGTCAGTGGAGAACAGATGAGTGATGTCGTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATGGCAATTGTTcag                                                                                          <  2:207805/251‑1 (MQ=21)                                                                                                                                                                            gATCAGATGTATAAAAAATTAAAATTAACCCCAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTAAGCAGAGCTTTGAAcc                                                                            >  2:318726/1‑251 (MQ=255)                                                                                                                                                                                       taAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCAAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACgg                                                                 >  1:119608/1‑251 (MQ=255)                                                                                                                                                                                             attaaaattaaCCACAATAAGCGAATTGATTAAAAATCTTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGCTTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTccc                                                           >  2:326438/1‑251 (MQ=255)                                                                                                                                                                                               taaaattaaCCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTc                                                         >  1:80220/1‑251 (MQ=255)                                                                                                                                                                                                                     aTTGATTTGAAATATTTATTGATCACTATCCGTTATAAGACTGGACCCCGGAAACACCAGCCAACCACTATAACTAAAATAAGCGATACTCTTAATACTAGCTTTTAGACTAGTCATTGGAGAACAGATTATTGATGTCTTAGCGCCGGAGAAACGAAGCCGGCGTACCACACAGGAAAAGATCGCAATTGTGCAGCAGAGCTATGAACCGGGAATGACGGTCTCCCTCGTTGCACGGCAACATAgtgt                                     >  2:180128/1‑249 (MQ=2)                                                                                                                                                                                                                               aaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGATTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCagccagcc                          >  1:217934/1‑250 (MQ=255)                                                                                                                                                                                                                                     tattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGAc                                                                                                                             <  2:109586/145‑1 (MQ=255)                                                                                                                                                                                                                                     tattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGAc                                                                                                                             >  1:109586/1‑145 (MQ=255)                                                                                                                                                                                                                                      attGTTCATTATCCGGTATTAGACTGGACCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGCTAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTATGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTAttt                  >  2:355642/1‑251 (MQ=255)                                                                                                                                                                                                                                       ttGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTtc                 >  1:228351/1‑251 (MQ=37)                                                                                                                                                                                                                                                      tattaGGCTGGCCCCCTGATTCTCCAAACAACCAATATCACTTAAATAAGTGATAGTCGTAATACTATTTTTTAGACGAGTGATTGGGGAAGAGATGATTGATGTCTTAGGGCCGTAGAAACGCAGACGACGCACCGCACAGGAAAAGAACGCAATTGTGCAGCAGAGCTTGGAACCAGGGATGACGGTCTCCCGCGTTGCCCGGCACCATGGTGTAGCAGCCCGCCAGTTATTTCTCTGGCGTAAGCaa   <  2:340175/250‑1 (MQ=11)                                                                                                                                                                                                                                                       attaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGCAAt  <  1:35453/250‑1 (MQ=11)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                        CAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGCAAT  >  NC_000913/3185848‑3186343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.