Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,105,376	IS2 (–) +5 bp	intergenic (+83/‑34)	csgC → / → ymdA	curlin chaperone/uncharacterized protein YmdA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	381260 =	NA (NA)	11 (0.320)	11/484	1.3	100%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	1105376 =	0 (0.000)					intergenic (+83/‑38)	csgC/ymdA	curlin chaperone/uncharacterized protein YmdA
*	?	NC_000913	= 1105380	0 (0.000)	22 (0.650)	22/486	0.5	100%	intergenic (+87/‑34)	csgC/ymdA	curlin chaperone/uncharacterized protein YmdA
	?	NC_000913	1467910 =	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

ACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/381472‑381260 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGAATGGTTCT  >  NC_000913/1105376‑1105593                                                                                                                                                                                                                                                                                                                                                                                                                                                 ACACCATGGTGCCGTGCAACGAGGGATACCGTCATCCCCGGTTCAAAGCTCGGCTGAACAATTGCGATCTTTTCCTGGGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCGCCAATGACTAGTCTAAAAACTAGTATTAAGACTAACACTTATTTATTTTATATTTGTTGTCTTAAGAGTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTAAGGAAATAGAA                                                                                                                                                                                         <  2:202218/248‑1          TGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAG                                                                                                                                                                              <  2:292601/250‑1                                          TTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTT                                                                                                                                             >  2:146029/1‑251                                           TCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTT                                                                                                                                             <  1:219232/250‑1                                                                                                              AGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAGATAGAATAACTGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAc                                                                          >  1:67184/1‑249                                                                                                                          TCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTCTCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGG                                                             >  2:158847/1‑251                                                                                                                              TTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATG                                                         >  1:195306/1‑251                                                                                                                                                          ATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTAGGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACCCGT                              <  2:36958/250‑1                                                                                                                                                                           TTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGAACCCAGTCATCACCCGTAGAAATGAACTGCCCAC             >  1:41375/1‑250                                                                                                                                                                                    TATTGGTTGTCTGGAGATTCAGGGGGCCAGGCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGAACTTTTTTAAACTCTCTTATGCTAGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTTACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGACCACAGAATGGTTt   >  1:64630/1‑250                                                                                                                                                                                     ATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGATCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGAATGGTTCT  >  2:120102/1‑251                                                                                                                                                                                                                                                                                                                                                                                                                                                 ACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/381472‑381260 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGAATGGTTCT  >  NC_000913/1105376‑1105593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.