Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,3630C.85.0% 55.2 / 7.5 20intergenic (‑490/+919)gatD/gatBgalactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base . (12/5);  total (13/7)
Fisher's exact test for biased strand distribution p-value = 2.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01
*NC_0009132,173,3640C.85.0% 55.3 / 7.3 20intergenic (‑491/+918)gatD/gatBgalactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base . (12/5);  total (13/7)
Fisher's exact test for biased strand distribution p-value = 2.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01

ATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTC  >  NC_000913/2173125‑2173612
                                                                                                                                                                                                                                              ||                                                                                                                                                                                                                                                        
aTTAGCCGCAGGTGGGGTGTGCAAACCAAGAGTTTGCGTCGCGATCCACAGGGGGAGGCTAAGAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGAGCGCCAAATGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGTGTTGCCGAGACCACC‑‑GCCGTATTTCCca                                                                                                                                                                                                                                             <  2:178065/251‑1 (MQ=255)
             tGGGTGTGCACACAAATGATTTGCGTCGCGATCCACAGGGTGATGCTCATAATGAAGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGAACGGCGACCGCCATCGCCACGCCGAAGCCGATGGTGGATAGATCGCCAAACGGCCGCACCTGATTACCCGGCACACAAACAGCACTTAAAATGGTGAGAGGGATAAAAATCAGGCTTGACGATACCACC‑‑GCCGTATGTCCCAGCTGCAACGCCgg                                                                                                                                                                                                                                >  2:226690/1‑251 (MQ=255)
             ggggTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCCCGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCg                                                                                                                                                                                                                                 <  1:183207/249‑1 (MQ=255)
                                tttGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACACGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGACGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCAAATCAGGAAc                                                                                                                                                                                                             >  1:105493/1‑251 (MQ=255)
                                     gTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTg                                                                                                                                                                                                        >  1:278247/1‑251 (MQ=255)
                                            tCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgccg                                                                                                                                                                                                 >  1:154756/1‑251 (MQ=255)
                                               acaGGGTGATGCTCATAATGAGGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGCCCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAAc                                                                                                                                                                                              <  1:24946/251‑1 (MQ=255)
                                                  gggTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTc                                                                                                                                                                                           >  2:186118/1‑251 (MQ=255)
                                                       aTGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTCCCACGATGCACGGCGCCCGCCATCGCCACGAAGAAGCGGATGTGGGCAAGATCGCCAAACGGCAGCACCGGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTGGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTg                                                                                                                                                                                      <  1:32578/251‑1 (MQ=255)
                                                                                     gTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTCTGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAc                                                                                                                                                                                                      >  2:321542/1‑205 (MQ=255)
                                                                                     gTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATAC‑‑CTCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAc                                                                                                                                                                                                      <  1:321542/205‑1 (MQ=255)
                                                                                         ggAACAGATTTCCACGATGCACGGCGACCGACACCACCATGAAGAAGCCCACGACGGCCAGATCGCAAATCGGCAGCACCTGATTCCCCGGCACACAAACAGCAAATAAAATGGTGAGTGGACTAAAAATCAGGATTGCCGATACAACC‑‑GCCGTATGTCCCAGAAGCCACGCAGGATCAAGGCCAATCAGGAACTACTGACCACCAACCTTAGACTGTACACCACTACGCGCCTGATTAGCGATGGGCgct                                                                                                                                                    >  1:153243/1‑249 (MQ=255)
                                                                                                   tCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCAAGCAGCAACACCGGATCAAGGCCAATCAGGCACTCCTGACCGCCGAACTTCGCCTGTAAACGACTCCGAGCCTGCTTAGCGATGGGCGTTAAAccatcc                                                                                                                                           >  1:240110/1‑250 (MQ=255)
                                                                                                                         aTCGCCACGAAGAAGCCGATGGCGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACAAAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACAGCCGAACTTCGCCTGTAAACGACTACGCGCCTGCTTAGCGATGGGCATTAAACCATCCATGATGGGTTTAATCACCCGTgg                                                                                                                    >  2:54599/1‑251 (MQ=255)
                                                                                                                                                                               cccGACACACAAACACCAATAAAAATGGGGAGTGGGAAAAAAACCAGGCTGGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACAGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCATTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGAc                                                              <  1:54599/251‑1 (MQ=255)
                                                                                                                                                                                                        tGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGCCATCGTAACCCGCGAGGATCCCGAt                                      >  2:107420/1‑250 (MQ=255)
                                                                                                                                                                                                         ggTGAGTGGGATAAAAATCAGGCTTACAGATA‑‑ACACCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTCGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATa                                     >  2:203273/1‑250 (MQ=255)
                                                                                                                                                                                                          gtgcgTGGGATAAAAATCAGGCTTGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGCACTTCGCCTGCCAACGACTACGAGCATGCTTAGCGAAGGGCGTTAAAACATCCATGATGGGTTTAATCACACGTGGCATTAGCAACATCACCGCAGCCGTTTTTACCGACAACTGAAATACACATTTGACATCGCAACCCCCGAGGATGCCgctac                                    >  2:287648/5‑246 (MQ=255)
                                                                                                                                                                                                              gTGGGAAAAAAACCGGGCTGGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGc                               <  1:186118/251‑1 (MQ=255)
                                                                                                                                                                                                                   aTAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAACCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCAt                          >  1:358112/1‑251 (MQ=255)
                                                                                                                                                                                                                                       gataccaccgccgTATGTCCCAGCAGCACCGCCGGATCAAGGACAATCAGCACCTCCCGACCCCCGAACTTCGCCTGTAAACGACTCCGAGCATGCTTAGCGATGGGCGTTAAACCATCCCTGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCACCGTTTTTACCGCCAGCTGCACTACACCTTTGACCTCGTAACCCGCGCGGAGGCCGATAATCAGCCCCATCACAAAACCCACGGTGAc        >  2:163437/8‑251 (MQ=255)
                                                                                                                                                                                                                                       gataccaccgccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCACTCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTgcc        >  2:150320/8‑249 (MQ=255)
                                                                                                                                                                                                                                       gataccaccgccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGAc        >  2:3411/8‑251 (MQ=255)
                                                                                                                                                                                                                                            caccgccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATAAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGCTGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCt   >  1:129617/3‑251 (MQ=255)
                                                                                                                                                                                                                                             cccGCCGTATGTCCCAGCAGCAACGCCGGACCAAGCCCAACCAGGAACTCCTGACCCCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAggatc  <  2:244583/251‑4 (MQ=255)
                                                                                                                                                                                                                                              ||                                                                                                                                                                                                                                                        
ATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTC  >  NC_000913/2173125‑2173612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑