Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,000,983	Δ1 bp	coding (42/552 nt)	fliZ ←	DNA‑binding transcriptional regulator FliZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,000,983	0	A	.	100.0%	62.2 / NA	15	coding (42/552 nt)	fliZ	DNA‑binding transcriptional regulator FliZ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (7/8);  total (7/8)

TATCGAAAAAATCACTCTGCGTTTTGCAATGTAAATCACCGCAAAATCGGCACAATGCCGCCCATGATTTTTGTTCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGCGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATAGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGTAATAGAGGGTTAATACCAGTTTTTCGCGCTCCGG  >  NC_000913/2000760‑2001222                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                tatCGAAAAAATCACTCTGCGTTTTGCAATGTAAATCACCGCAAAATCGGCACAATGCCGCCCATGATTTTTGTTCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGCGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAG‑TAGCGGCTTAATGGCCGTCTTTTCAGGt                                                                                                                                                                                                                     >  1:344668/1‑251 (MQ=255)            tCACTCTGCGTTTTGCAATGTAAATCACCGCAAAATCGGCACAATGCCGCCCATGATTTTTGTTCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGCGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACTAAGGTAATGAGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAG‑TAGCGGCTTAATGGCCGTCTTTTCAGGTGTTGCACcatc                                                                                                                                                                                                          >  2:190540/1‑251 (MQ=255)                   gcggtttGCAATGTAAATCACCGCAAAAGCGGCACAATGCCGCCCATGATTTTTGTTCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGCGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAG‑TAGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGa                                                                                                                                                                                                   <  2:245682/247‑1 (MQ=255)                                              aTCGGCACAATGCCGCCCATGATTTTTGTTCCGTTTGCAAGCCATTTTCATCAAGCAGCGTGTCCAGGCGGGAAATCTCGATTTTATTCACTATTTTGCCGGCGCGAACTAAGGTAAGGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAG‑TAGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCg                                                                                                                                                                        <  2:74529/251‑1 (MQ=255)                                                       aTGCCGCCCATGATTTTTGTTCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGCGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAG‑TAGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACt                                                                                                                                                               >  1:107004/1‑251 (MQ=255)                                                           cgccCATGATTTTTGTTCCGTATGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGCGGGAAATCTCGCTTTTATTCCCTATTTTGCCGTCACGCACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCCATGGGTCTGGCTGTGTTTAAAGTCTTTAAG‑GAGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCAACATTAAAAACTCCTGTGAGTCAACGTTAAAGTGGGGCATTTACtaccg                                                                                                                                                           >  2:59979/1‑247 (MQ=255)                                                                      tttGTTCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGCGGGAAATCTCGATTTTATTCACTAGTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAG‑TAGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTGAAAGTGCGGCATTTACTGACGTTATAACTTa                                                                                                                                                 <  1:356168/250‑1 (MQ=255)                                                                                      gccaTTTTCATCAAGCAGCGTGTCCAGGCGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAG‑TAGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTaa                                                                                                                                <  1:276631/251‑1 (MQ=255)                                                                                         aTTTTCATCAAGCAGCGTGTCCAGGCGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAG‑TAGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGGGCGTAACCg                                                                                                                             >  2:248423/1‑251 (MQ=255)                                                                                                                                   aTTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAG‑TAGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGGCTGACCCGCGATTccc                                                                                    <  1:316401/250‑1 (MQ=255)                                                                                                                                                                    gCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAG‑TAGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGAt                                                   <  1:263443/250‑1 (MQ=255)                                                                                                                                                                      gATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAG‑TAGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCAGTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCa                                                <  2:107004/251‑1 (MQ=255)                                                                                                                                                                                    cGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAG‑TAGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGTaa                                  <  1:248423/251‑1 (MQ=255)                                                                                                                                                                                        aaTGCGTGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAG‑TAGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGTAAt                                 >  1:101348/1‑248 (MQ=255)                                                                                                                                                                                                                    aaaGTCTTTAAG‑TAGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCCCCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGTAATAGAGGGTTAATACCAGTTTTTCGCGCTCCgg  >  2:207858/1‑251 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                TATCGAAAAAATCACTCTGCGTTTTGCAATGTAAATCACCGCAAAATCGGCACAATGCCGCCCATGATTTTTGTTCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGCGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATAGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTACCCAGTTTAGTGCGTAACCGTTTAATAGCCTGGCTGTGTAACTGACTGACCCGCGATTCCCCGACCTCCAGCACCGCGCCAATCTCTTTGAGATTCAGCTCTTCCTGGTAATAGAGGGTTAATACCAGTTTTTCGCGCTCCGG  >  NC_000913/2000760‑2001222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑