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Mutants (Isolated)

Allele Name		tm545
Allele Type		Normal
Sequence Name		Y66H1B.3
Gene Name		fln-1
Worm Base	Allele Name	tm545
	Gene Name	fln-1
	Sequence	Y66H1B.3
Phenotype Information from the receiver is posted in the form of a "researcher : phenotype"		homozygous viable. Dr. D. Greenstein: low brood size at 20C.
Mutation site Please see gene structure to locate the deletion in relation to exon(s)		26266/26267-TCAGTAAAATTTGAATA-26529/26530 (263 bp deletion + 17 bp insertion)
Chromosome		IV
Putative gene structure		complement(join(21251..21978, 22294..22393, 22461..23015, 23696..24231, 24532..24808, 24850..25412, 25459..25632, 26482..26653, 26702..26815, 26865..26900))
Map position		-26.37
Balancer
Map position of balancer
Sequence of primers		ExtRev:GTTTTCAGTGTATGGGAGCT,IntFwd:GCCACAGTTGGTGTATAGGA,ExtFwd:CCCAACTTTGTAAACTCCAG,IntRev:GGAGTTCCCCTTTCCTCTAT
Distributed lab
Depositor		Dr. S. Mitani
References		Please submit your publication Kelley CA, Triplett O, Mallick S, Burkewitz K, Mair WB, Cram EJ. FLN-1/filamin is required to anchor the actomyosin cytoskeleton and for global organization of sub-cellular organelles in a contractile tissue. Cytoskeleton (Hoboken) 2020 77(10) 379-398  [ PubMed ID = 32969593 ] [ RRC reference ] Choi S, Ambros V. The C. elegans heterochronic gene lin-28 coordinates the timing of hypodermal and somatic gonadal programs for hermaphrodite reproductive system morphogenesis. Development 2019 146(5)   [ PubMed ID = 30745431 ] [ RRC reference ] Kovacevic I, Cram EJ. FLN-1/filamin is required for maintenance of actin and exit of fertilized oocytes from the spermatheca in C. elegans. Dev Biol 2010 347(2) 247-57  [ PubMed ID = 20707996 ] [ RRC reference ] Kovacevic I, Orozco JM, Cram EJ. Filamin and phospholipase C-ε are required for calcium signaling in the Caenorhabditis elegans spermatheca. PLoS Genet 2013 9(5) e1003510  [ PubMed ID = 23671426 ] [ RRC reference ] Nakamura F, Kumeta K, Hida T, Isono T, Nakayama Y, Kuramata-Matsuoka E, Yamashita N, Uchida Y, Ogura K, Gengyo-Ando K, Mitani S, Ogino T, Goshima Y. Amino- and carboxyl-terminal domains of Filamin-A interact with CRMP1 to mediate Sema3A signalling. Nat Commun 2014 5 5325  [ PubMed ID = 25358863 ] [ RRC reference ]