Mutants (Isolated)

tm3100

Allele Nametm3100
Sequence NameC09G5.8
CGC NameC09G5.8
Worm BaseAllele Name tm3100
CGC Name C09G5.8
Sequence C09G5.8
Phenotypehomozygous viable. Dr. O. Blacque: Dyf (+). Dr. K. Ashrafi: non-Gro.
Mutation site26722/26723-27262/27263 (540 bp deletion)
ChromosomeII
Putative gene structurejoin(24056..24159, 24467..24576, 24694..24835, 24885..24951, 25049..25173, 26121..26246, 26614..26913, 26993..27125, 27180..27547, 27861..28121, 29490..29688, Z46792.1:109..204, Z46792.1:256..426, Z46792.1:664..882, Z46792.1:1065..1283, Z46792.1:2577..2846, Z46792.1:3000..3449, Z46792.1:3497..3600, Z46792.1:3647..4197, Z46792.1:4282..4608, Z46792.1:5030..5200, Z46792.1:5252..5334)
Map position2.84
Balancer
Map position of balancer
Sequence of primersExtFwd:CGGCATAGGGAGAGATAATA,IntFwd:AATTCCAGGGTATCCGCAAG,ExtRev:GATCTTCACGACGTCTGTGT,IntRev:ATACTTTCCCGTGCTCCGTA
Distributed lab
DepositorDr. S. Mitani/NBRP
References Please submit your publication
Jensen VL, Li C, Bowie RV, Clarke L, Mohan S, Blacque OE, Leroux MR.
Formation of the transition zone by Mks5/Rpgrip1L establishes a ciliary zone of exclusion (CIZE) that compartmentalises ciliary signalling proteins and controls PIP2 ciliary abundance.
EMBO J. 2015 34(20) 2537-56 
[ PubMed ID = 26392567 ] [ RRC reference ]

Nguyen PA, Liou W, Hall DH, Leroux MR.
Ciliopathy proteins establish a bipartite signaling compartment in a C. elegans thermosensory neuron.
J. Cell. Sci. 2014 127(Pt 24) 5317-30 
[ PubMed ID = 25335890 ] [ RRC reference ]

Williams CL, Li C, Kida K, Inglis PN, Mohan S, Semenec L, Bialas NJ, Stupay RM, Chen N, Blacque OE, Yoder BK, Leroux MR.
MKS and NPHP modules cooperate to establish basal body/transition zone membrane associations and ciliary gate function during ciliogenesis.
J. Cell Biol. 2011 192(6) 1023-41 
[ PubMed ID = 21422230 ] [ RRC reference ]

Liu L, Zhang M, Xia Z, Xu P, Chen L, Xu T.
Caenorhabditis elegans ciliary protein NPHP-8, the homologue of human RPGRIP1L, is required for ciliogenesis and chemosensation.
Biochem. Biophys. Res. Commun. 2011 410(3) 626-31 
[ PubMed ID = 21689635 ] [ RRC reference ]

Roberson EC, Dowdle WE, Ozanturk A, Garcia-Gonzalo FR, Li C, Halbritter J, Elkhartoufi N, Porath JD, Cope H, Ashley-Koch A, Gregory S, Thomas S, Sayer JA, Saunier S, Otto EA, Katsanis N, Davis EE, Attié-Bitach T, Hildebrandt F, Leroux MR, Reiter JF.
TMEM231, mutated in orofaciodigital and Meckel syndromes, organizes the ciliary transition zone.
J. Cell Biol. 2015 209(1) 129-42 
[ PubMed ID = 25869670 ] [ RRC reference ]

Cevik S, Sanders AA, Van Wijk E, Boldt K, Clarke L, van Reeuwijk J, Hori Y, Horn N, Hetterschijt L, Wdowicz A, Mullins A, Kida K, Kaplan OI, van Beersum SE, Man Wu K, Letteboer SJ, Mans DA, Katada T, Kontani K, Ueffing M, Roepman R, Kremer H, Blacque OE.
Active transport and diffusion barriers restrict Joubert Syndrome-associated ARL13B/ARL-13 to an Inv-like ciliary membrane subdomain.
PLoS Genet. 2013 9(12) e1003977 
[ PubMed ID = 24339792 ] [ RRC reference ]

Huang L, Szymanska K, Jensen VL, Janecke AR, Innes AM, Davis EE, Frosk P, Li C, Willer JR, Chodirker BN, Greenberg CR, McLeod DR, Bernier FP, Chudley AE, Müller T, Shboul M, Logan CV, Loucks CM, Beaulieu CL, Bowie RV, Bell SM, Adkins J, Zuniga FI, Ross KD, Wang J, Ban MR, Becker C, Nürnberg P, Douglas S, Craft CM, Akimenko MA, Hegele RA, Ober C, Utermann G, Bolz HJ, Bulman DE, Katsanis N, Blacque OE, Doherty D, Parboosingh JS, Leroux MR, Johnson CA, Boycott KM.
TMEM237 is mutated in individuals with a Joubert syndrome related disorder and expands the role of the TMEM family at the ciliary transition zone.
Am. J. Hum. Genet. 2011 89(6) 713-30 
[ PubMed ID = 22152675 ] [ RRC reference ]

Jensen VL, Carter S, Sanders AA, Li C, Kennedy J, Timbers TA, Cai J, Scheidel N, Kennedy BN, Morin RD, Leroux MR, Blacque OE.
Whole-Organism Developmental Expression Profiling Identifies RAB-28 as a Novel Ciliary GTPase Associated with the BBSome and Intraflagellar Transport.
PLoS Genet. 2016 12(12) e1006469 
[ PubMed ID = 27930654 ] [ RRC reference ]

Brear AG, Yoon J, Wojtyniak M, Sengupta P.
Diverse cell type-specific mechanisms localize G protein-coupled receptors to Caenorhabditis elegans sensory cilia.
Genetics 2014 197(2) 667-84 
[ PubMed ID = 24646679 ] [ RRC reference ]