Mutants (Isolated)

tm2182

Allele Nametm2182
Sequence NameR12B2.5
CGC Namemdt-15
Worm BaseAllele Name tm2182
CGC Name mdt-15
Sequence R12B2.5
Phenotypehomozygous viable. Dr. K.R. Yamamoto: PLoS Genetics 4, e1000021 (2008).
Mutation site29157/29158-29635/29636 (478 bp deletion)
ChromosomeIII
Putative gene structurecomplement(join(28044..28142, 28221..28538, 28889..30043, 30116..30597, 30657..30880, 31104..31159))
Map position-1.44
Balancer
Map position of balancer
Sequence of primersIntRev:CCATAGGAGCTGATGTCACA,ExtFwd:CACCCATTGATCCAGGTGTT,ExtRev:CCTTCGCCGAAATTTCGAGA,IntFwd:AGGGGCTCCAGAACTTGGAC
Distributed lab
DepositorDr. S. Mitani/NBRP
References Please submit your publication
Taubert S, Hansen M, Van Gilst MR, Cooper SB, Yamamoto KR.
The Mediator subunit MDT-15 confers metabolic adaptation to ingested material.
PLoS Genet. 2008 4(2) e1000021 
[ PubMed ID = 18454197 ] [ RRC reference ]

Hou NS, Gutschmidt A, Choi DY, Pather K, Shi X, Watts JL, Hoppe T, Taubert S.
Activation of the endoplasmic reticulum unfolded protein response by lipid disequilibrium without disturbed proteostasis in vivo.
Proc. Natl. Acad. Sci. U.S.A. 2014 111(22) E2271-80 
[ PubMed ID = 24843123 ] [ RRC reference ]

Pang S, Lynn DA, Lo JY, Paek J, Curran SP.
SKN-1 and Nrf2 couples proline catabolism with lipid metabolism during nutrient deprivation.
Nat Commun 2014 5 5048 
[ PubMed ID = 25284427 ] [ RRC reference ]

Pukkila-Worley R, Feinbaum RL, McEwan DL, Conery AL, Ausubel FM.
The evolutionarily conserved mediator subunit MDT-15/MED15 links protective innate immune responses and xenobiotic detoxification.
PLoS Pathog. 2014 10(5) e1004143 
[ PubMed ID = 24875643 ] [ RRC reference ]

Qiao Y, Zhao Y, Wu Q, Sun L, Ruan Q, Chen Y, Wang M, Duan J, Wang D.
Full toxicity assessment of Genkwa Flos and the underlying mechanism in nematode Caenorhabditis elegans.
PLoS ONE 2014 9(3) e91825 
[ PubMed ID = 24626436 ] [ RRC reference ]

Wu QL, Rui Q, He KW, Shen LL, Wang DY.
UNC-64 and RIC-4, the plasma membrane-associated SNAREs syntaxin and SNAP-25, regulate fat storage in nematode Caenorhabditis elegans.
Neurosci Bull 2010 26(2) 104-16 
[ PubMed ID = 20332815 ] [ RRC reference ]

Schleit J, Wall VZ, Simko M, Kaeberlein M.
The MDT-15 subunit of mediator interacts with dietary restriction to modulate longevity and fluoranthene toxicity in Caenorhabditis elegans.
PLoS ONE 2011 6(11) e28036 
[ PubMed ID = 22132200 ] [ RRC reference ]

Goh GY, Martelli KL, Parhar KS, Kwong AW, Wong MA, Mah A, Hou NS, Taubert S.
The conserved Mediator subunit MDT-15 is required for oxidative stress responses in Caenorhabditis elegans.
Aging Cell 2014 13(1) 70-9 
[ PubMed ID = 23957350 ] [ RRC reference ]

Han S, Schroeder EA, Silva-García CG, Hebestreit K, Mair WB, Brunet A.
Mono-unsaturated fatty acids link H3K4me3 modifiers to C. elegans lifespan.
Nature 2017 544(7649) 185-190 
[ PubMed ID = 28379943 ] [ RRC reference ]

Kniazeva M, Zhu H, Sewell AK, Han M.
A Lipid-TORC1 Pathway Promotes Neuronal Development and Foraging Behavior under Both Fed and Fasted Conditions in C. elegans.
Dev. Cell 2015 33(3) 260-71 
[ PubMed ID = 25892013 ] [ RRC reference ]

Steimel A, Suh J, Hussainkhel A, Deheshi S, Grants JM, Zapf R, Moerman DG, Taubert S, Hutter H.
The C. elegans CDK8 Mediator module regulates axon guidance decisions in the ventral nerve cord and during dorsal axon navigation.
Dev. Biol. 2013 377(2) 385-98 
[ PubMed ID = 23458898 ] [ RRC reference ]