Mutants (Isolated)

tm2026

Allele Nametm2026
Sequence NameW03A3.2
CGC Namepolq-1
Worm BaseAllele Name tm2026
CGC Name polq-1
Sequence W03A3.2
Phenotypehomozygous viable
Mutation site8943/8944-9855/9856 (912 bp deletion)
ChromosomeIII
Putative gene structurecomplement(join(3560..3849, 4228..4437, 4489..4822, 4944..5612, 5668..5825, 6090..6318, 6365..6468, 6695..7452, 8474..8578, 9009..9147, 9281..9350, 9541..9774, 9829..9906, 10014..10397, 10819..10952, 13612..13734, 14205..14847, 14898..[R12B2]568, 621..744, 795..863))
Map position-1.45
Balancer
Map position of balancer
Sequence of primersExtFwd:ACAGACACGTCACGCCACGT,IntFwd:CACGCCACGTTCATGTAGGA,ExtRev:CGAGATGCTGAACGGACTAA,IntRev:TTGCTAAAGGGCGTGCGCCT
Distributed lab
DepositorDr. S. Mitani/NBRP
References Please submit your publication
Kamp JA, van Schendel R, Dilweg IW, Tijsterman M.
BRCA1-associated structural variations are a consequence of polymerase theta-mediated end-joining.
Nat Commun 2020 11(1) 3615 
[ PubMed ID = 32680986 ] [ RRC reference ]

van Bostelen I, van Schendel R, Romeijn R, Tijsterman M.
Translesion synthesis polymerases are dispensable for C. elegans reproduction but suppress genome scarring by polymerase theta-mediated end joining.
PLoS Genet 2020 16(4) e1008759 
[ PubMed ID = 32330130 ] [ RRC reference ]

van Bostelen I, Tijsterman M.
Combined loss of three DNA damage response pathways renders C. elegans intolerant to light.
DNA Repair (Amst) 2017 54 55-62 
[ PubMed ID = 28472716 ] [ RRC reference ]

Hefel A, Honda M, Cronin N, Harrell K, Patel P, Spies M, Smolikove S.
RPA complexes in Caenorhabditis elegans meiosis; unique roles in replication, meiotic recombination and apoptosis.
Nucleic Acids Res 2021 49(4) 2005-2026 
[ PubMed ID = 33476370 ] [ RRC reference ]

Lemmens B, van Schendel R, Tijsterman M.
Mutagenic consequences of a single G-quadruplex demonstrate mitotic inheritance of DNA replication fork barriers.
Nat Commun 2015 6 8909 
[ PubMed ID = 26563448 ] [ RRC reference ]

van Schendel R, van Heteren J, Welten R, Tijsterman M.
Genomic Scars Generated by Polymerase Theta Reveal the Versatile Mechanism of Alternative End-Joining.
PLoS Genet 2016 12(10) e1006368 
[ PubMed ID = 27755535 ] [ RRC reference ]

Roerink SF, van Schendel R, Tijsterman M.
Polymerase theta-mediated end joining of replication-associated DNA breaks in C. elegans.
Genome Res 2014 24(6) 954-62 
[ PubMed ID = 24614976 ] [ RRC reference ]

Bertolini S, Wang B, Meier B, Hong Y, Gartner A.
Caenorhabditis elegans BUB-3 and SAN-1/MAD3 Spindle Assembly Checkpoint Components Are Required for Genome Stability in Response to Treatment with Ionizing Radiation.
G3 (Bethesda) 2017 7(12) 3875-3885 
[ PubMed ID = 29046436 ] [ RRC reference ]

González-Huici V, Wang B, Gartner A.
A Role for the Nonsense-Mediated mRNA Decay Pathway in Maintaining Genome Stability in Caenorhabditis elegans.
Genetics 2017 206(4) 1853-1864 
[ PubMed ID = 28634159 ] [ RRC reference ]

Macaisne N, Kessler Z, Yanowitz JL.
Meiotic Double-Strand Break Proteins Influence Repair Pathway Utilization.
Genetics 2018 210(3) 843-856 
[ PubMed ID = 30242011 ] [ RRC reference ]

Ye FB, Hamza A, Singh T, Flibotte S, Hieter P, O'Neil NJ.
A Multimodal Genotoxic Anticancer Drug Characterized by Pharmacogenetic Analysis in Caenorhabditis elegans.
Genetics 2020 215(3) 609-621 
[ PubMed ID = 32414869 ] [ RRC reference ]

Koole W, van Schendel R, Karambelas AE, van Heteren JT, Okihara KL, Tijsterman M.
A Polymerase Theta-dependent repair pathway suppresses extensive genomic instability at endogenous G4 DNA sites.
Nat Commun 2014 5 3216 
[ PubMed ID = 24496117 ] [ RRC reference ]

van Schendel R, Roerink SF, Portegijs V, van den Heuvel S, Tijsterman M.
Polymerase Θ is a key driver of genome evolution and of CRISPR/Cas9-mediated mutagenesis.
Nat Commun 2015 6 7394 
[ PubMed ID = 26077599 ] [ RRC reference ]

Kessler Z, Yanowitz J.
Methodological considerations for mutagen exposure in C. elegans.
Methods 2014 68(3) 441-9 
[ PubMed ID = 24768858 ] [ RRC reference ]

Muzzini DM, Plevani P, Boulton SJ, Cassata G, Marini F.
Caenorhabditis elegans POLQ-1 and HEL-308 function in two distinct DNA interstrand cross-link repair pathways.
DNA Repair (Amst) 2008 7(6) 941-50 
[ PubMed ID = 18472307 ] [ RRC reference ]