Mutants (Isolated)

tm2026

Allele Nametm2026
Sequence NameW03A3.2
CGC Namepolq-1
Worm BaseAllele Name tm2026
CGC Name polq-1
Sequence W03A3.2
Phenotypehomozygous viable
Mutation site8943/8944-9855/9856 (912 bp deletion)
ChromosomeIII
Putative gene structurecomplement(join(3668..3849, 4228..4437, 4489..4822, 4944..5612, 5668..5825, 6090..6318, 6365..6468, 6695..7452, 8474..8578, 9009..9147, 9281..9350, 9541..9774, 9829..9906, 10014..10292))
Map position-1.45
Balancer
Map position of balancer
Sequence of primersExtFwd:ACAGACACGTCACGCCACGT,IntFwd:CACGCCACGTTCATGTAGGA,ExtRev:CGAGATGCTGAACGGACTAA,IntRev:TTGCTAAAGGGCGTGCGCCT
Distributed lab
DepositorDr. S. Mitani/NBRP
References Please submit your publication
van Bostelen I, Tijsterman M.
Combined loss of three DNA damage response pathways renders C. elegans intolerant to light.
DNA Repair (Amst.) 2017 54 55-62 
[ PubMed ID = 28472716 ] [ RRC reference ]

Roerink SF, van Schendel R, Tijsterman M.
Polymerase theta-mediated end joining of replication-associated DNA breaks in C. elegans.
Genome Res. 2014 24(6) 954-62 
[ PubMed ID = 24614976 ] [ RRC reference ]

González-Huici V, Wang B, Gartner A.
A Role for the Nonsense-Mediated mRNA Decay Pathway in Maintaining Genome Stability in Caenorhabditis elegans.
Genetics 2017 206(4) 1853-1864 
[ PubMed ID = 28634159 ] [ RRC reference ]

Bertolini S, Wang B, Meier B, Hong Y, Gartner A.
Caenorhabditis elegans BUB-3 and SAN-1/MAD3 Spindle Assembly Checkpoint Components Are Required for Genome Stability in Response to Treatment with Ionizing Radiation.
G3 (Bethesda) 2017 7(12) 3875-3885 
[ PubMed ID = 29046436 ] [ RRC reference ]

van Schendel R, Roerink SF, Portegijs V, van den Heuvel S, Tijsterman M.
Polymerase Θ is a key driver of genome evolution and of CRISPR/Cas9-mediated mutagenesis.
Nat Commun 2015 6 7394 
[ PubMed ID = 26077599 ] [ RRC reference ]

Koole W, van Schendel R, Karambelas AE, van Heteren JT, Okihara KL, Tijsterman M.
A Polymerase Theta-dependent repair pathway suppresses extensive genomic instability at endogenous G4 DNA sites.
Nat Commun 2014 5 3216 
[ PubMed ID = 24496117 ] [ RRC reference ]

Muzzini DM, Plevani P, Boulton SJ, Cassata G, Marini F.
Caenorhabditis elegans POLQ-1 and HEL-308 function in two distinct DNA interstrand cross-link repair pathways.
DNA Repair (Amst.) 2008 7(6) 941-50 
[ PubMed ID = 18472307 ] [ RRC reference ]

Kessler Z, Yanowitz J.
Methodological considerations for mutagen exposure in C. elegans.
Methods 2014 68(3) 441-9 
[ PubMed ID = 24768858 ] [ RRC reference ]