Mutants (Isolated)

tm1637

Allele Name

tm1637

Sequence Name

C04F12.1

CGC Name

C04F12.1

Worm Base

Allele Name

tm1637

CGC Name

C04F12.1

Sequence

C04F12.1

Phenotype

homozygous viable. Dr. C. Mello, Cell 127, 747-457 (2006). Dr. R. Lu: PLoS Pathogens 5, e1000286 (2009).

Mutation site

3597/3598-4395/4396 (798 bp deletion)

Chromosome

Putative gene structure

complement(join(1623..1740, 1791..2082, 2431..2770, 3173..3287, 3488..3574, 3626..3780, 3827..4069, 4115..4683, 4821..5651, 6179..6263))

Map position

3.85

Balancer

Map position of balancer

Sequence of primers

IntFwd:AACGCAGCTTGGCTCGTCAC,ExtFwd:CGCGCCTAATCCATGCTATC,IntRev:TCGTTACGACGTCAGTGTTC,ExtRev:CGAGTACGAACCTCCAGCAC

Distributed lab

Depositor

Dr. S. Mitani

References

Please submit your publication

Vasale JJ, Gu W, Thivierge C, Batista PJ, Claycomb JM, Youngman EM, Duchaine TF, Mello CC, Conte D Jr.
Sequential rounds of RNA-dependent RNA transcription drive endogenous small-RNA biogenesis in the ERGO-1/Argonaute pathway.
Proc Natl Acad Sci U S A 2010 107(8) 3582-7
[ PubMed ID = 20133583 ] [ RRC reference ]

Corrêa RL, Steiner FA, Berezikov E, Ketting RF.
MicroRNA-directed siRNA biogenesis in Caenorhabditis elegans.
PLoS Genet 2010 6(4) e1000903
[ PubMed ID = 20386745 ] [ RRC reference ]

Wu X, Shi Z, Cui M, Han M, Ruvkun G.
Repression of germline RNAi pathways in somatic cells by retinoblastoma pathway chromatin complexes.
PLoS Genet 2012 8(3) e1002542
[ PubMed ID = 22412383 ] [ RRC reference ]

Luteijn MJ, van Bergeijk P, Kaaij LJ, Almeida MV, Roovers EF, Berezikov E, Ketting RF.
Extremely stable Piwi-induced gene silencing in Caenorhabditis elegans.
EMBO J 2012 31(16) 3422-30
[ PubMed ID = 22850670 ] [ RRC reference ]

Kennedy LM, Grishok A.
Neuronal migration is regulated by endogenous RNAi and chromatin-binding factor ZFP-1/AF10 in Caenorhabditis elegans.
Genetics 2014 197(1) 207-20
[ PubMed ID = 24558261 ] [ RRC reference ]

Gu W, Shirayama M, Conte D Jr, Vasale J, Batista PJ, Claycomb JM, Moresco JJ, Youngman EM, Keys J, Stoltz MJ, Chen CC, Chaves DA, Duan S, Kasschau KD, Fahlgren N, Yates JR 3rd, Mitani S, Carrington JC, Mello CC.
Distinct argonaute-mediated 22G-RNA pathways direct genome surveillance in the C. elegans germline.
Mol Cell 2009 36(2) 231-44
[ PubMed ID = 19800275 ] [ RRC reference ]

She X, Xu X, Fedotov A, Kelly WG, Maine EM.
Regulation of heterochromatin assembly on unpaired chromosomes during Caenorhabditis elegans meiosis by components of a small RNA-mediated pathway.
PLoS Genet 2009 5(8) e1000624
[ PubMed ID = 19714217 ] [ RRC reference ]

Lu R, Yigit E, Li WX, Ding SW.
An RIG-I-Like RNA helicase mediates antiviral RNAi downstream of viral siRNA biogenesis in Caenorhabditis elegans.
PLoS Pathog 2009 5(2) e1000286
[ PubMed ID = 19197349 ] [ RRC reference ]