Mutants (Isolated)

tm1637

Allele Nametm1637
Sequence NameC04F12.1
CGC NameC04F12.1
Worm BaseAllele Name tm1637
CGC Name C04F12.1
Sequence C04F12.1
Phenotypehomozygous viable. Dr. C. Mello, Cell 127, 747-457 (2006). Dr. R. Lu: PLoS Pathogens 5, e1000286 (2009).
Mutation site3597/3598-4395/4396 (798 bp deletion)
ChromosomeI
Putative gene structurecomplement(join(1623..1740, 1791..2082, 2431..2770, 3173..3287, 3488..3574, 3626..3780, 3827..4069, 4115..4683, 4821..5651, 6179..6263))
Map position3.85
Balancer
Map position of balancer
Sequence of primersIntFwd:AACGCAGCTTGGCTCGTCAC,ExtFwd:CGCGCCTAATCCATGCTATC,IntRev:TCGTTACGACGTCAGTGTTC,ExtRev:CGAGTACGAACCTCCAGCAC
Distributed lab
DepositorDr. S. Mitani
References Please submit your publication
Kennedy LM, Grishok A.
Neuronal migration is regulated by endogenous RNAi and chromatin-binding factor ZFP-1/AF10 in Caenorhabditis elegans.
Genetics 2014 197(1) 207-20 
[ PubMed ID = 24558261 ] [ RRC reference ]

Lu R, Yigit E, Li WX, Ding SW.
An RIG-I-Like RNA helicase mediates antiviral RNAi downstream of viral siRNA biogenesis in Caenorhabditis elegans.
PLoS Pathog. 2009 5(2) e1000286 
[ PubMed ID = 19197349 ] [ RRC reference ]

Luteijn MJ, van Bergeijk P, Kaaij LJ, Almeida MV, Roovers EF, Berezikov E, Ketting RF.
Extremely stable Piwi-induced gene silencing in Caenorhabditis elegans.
EMBO J. 2012 31(16) 3422-30 
[ PubMed ID = 22850670 ] [ RRC reference ]

Wu X, Shi Z, Cui M, Han M, Ruvkun G.
Repression of germline RNAi pathways in somatic cells by retinoblastoma pathway chromatin complexes.
PLoS Genet. 2012 8(3) e1002542 
[ PubMed ID = 22412383 ] [ RRC reference ]

CorrĂȘa RL, Steiner FA, Berezikov E, Ketting RF.
MicroRNA-directed siRNA biogenesis in Caenorhabditis elegans.
PLoS Genet. 2010 6(4) e1000903 
[ PubMed ID = 20386745 ] [ RRC reference ]

Gu W, Shirayama M, Conte D Jr, Vasale J, Batista PJ, Claycomb JM, Moresco JJ, Youngman EM, Keys J, Stoltz MJ, Chen CC, Chaves DA, Duan S, Kasschau KD, Fahlgren N, Yates JR 3rd, Mitani S, Carrington JC, Mello CC.
Distinct argonaute-mediated 22G-RNA pathways direct genome surveillance in the C. elegans germline.
Mol. Cell 2009 36(2) 231-44 
[ PubMed ID = 19800275 ] [ RRC reference ]

She X, Xu X, Fedotov A, Kelly WG, Maine EM.
Regulation of heterochromatin assembly on unpaired chromosomes during Caenorhabditis elegans meiosis by components of a small RNA-mediated pathway.
PLoS Genet. 2009 5(8) e1000624 
[ PubMed ID = 19714217 ] [ RRC reference ]

Vasale JJ, Gu W, Thivierge C, Batista PJ, Claycomb JM, Youngman EM, Duchaine TF, Mello CC, Conte D Jr.
Sequential rounds of RNA-dependent RNA transcription drive endogenous small-RNA biogenesis in the ERGO-1/Argonaute pathway.
Proc. Natl. Acad. Sci. U.S.A. 2010 107(8) 3582-7 
[ PubMed ID = 20133583 ] [ RRC reference ]