Mutants (Isolated)

tm1489

Allele Nametm1489
Allele TypeNormal
Sequence NameK01G5.2a
Gene Namehpl-2
Worm BaseAllele Name tm1489
Gene Name hpl-2
Sequence K01G5.2a
Phenotype Information from the receiver is posted in the form of a "researcher : phenotype" homozygous viable. Dr. R.H. Horvitz: not completely penetrant multivulva and sterile at 25 degree, synthetic multivulva with mutations in class A, B and C genes. Dr. M. Han: WT at 20 degree, partial Muv at 25 degree. tm1489; lin-8 (null) is partial Muv at 20C, complete and obvious Muv at 25C. tm1489; lin-15A (RNAi) is Muv at 20C. Dr. W. Vermeulen: hpl-1 (tm1624) hpl-2 (tm1489) double mutant shows severe UV sensitivity.
Mutation site Please see gene structure to locate the deletion in relation to exon(s) 14347/14348-15019/15020 (672 bp deletion)
ChromosomeIII
Putative gene structurecomplement(join(13194..13266, 13966..14204, 14472..14645, 14920..14961))
Map position5.24
Balancer
Map position of balancer
Sequence of primersExtFwd:GGCACTTATGTGCAGTACAG,ExtRev:TACATGCAACAAACCGCGCT,IntFwd:GTGCAGTACAGCCGGCATAT,IntRev:TCAGAGCTCCTAACCTACCT
Distributed lab
DepositorDr. S. Mitani/NBRP
References Please submit your publication
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H3K9me1/2 methylation limits the lifespan of daf-2 mutants in C. elegans.
Elife 2022 11  
[ PubMed ID = 36125117 ] [ RRC reference ]

Bujarrabal-Dueso A, Sendtner G, Meyer DH, Chatzinikolaou G, Stratigi K, Garinis GA, Schumacher B.
The DREAM complex functions as conserved master regulator of somatic DNA-repair capacities.
Nat Struct Mol Biol 2023 30(4) 475-488 
[ PubMed ID = 36959262 ] [ RRC reference ]

Rodriguez-Crespo D, Nanchen M, Rajopadhye S, Wicky C.
The zinc-finger transcription factor LSL-1 is a major regulator of the germline transcriptional program in Caenorhabditis elegans.
Genetics 2022 221(1)  
[ PubMed ID = 35262739 ] [ RRC reference ]

Spichal M, Heestand B, Billmyre KK, Frenk S, Mello CC, Ahmed S.
Germ granule dysfunction is a hallmark and mirror of Piwi mutant sterility.
Nat Commun 2021 12(1) 1420 
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Hsu TY, Zhang B, L'Etoile ND, Juang BT.
C. elegans orthologs MUT-7/CeWRN-1 of Werner syndrome protein regulate neuronal plasticity.
Elife 2021 10  
[ PubMed ID = 33646120 ] [ RRC reference ]

Rechtsteiner A, Costello ME, Egelhofer TA, Garrigues JM, Strome S, Petrella LN.
Repression of Germline Genes in Caenorhabditis elegans Somatic Tissues by H3K9 Dimethylation of Their Promoters.
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[ PubMed ID = 30910798 ] [ RRC reference ]

Fields BD, Kennedy S.
Chromatin Compaction by Small RNAs and the Nuclear RNAi Machinery in C. elegans.
Sci Rep 2019 9(1) 9030 
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Saldi TK, Gonzales P, Garrido-Lecca A, Dostal V, Roberts CM, Petrucelli L, Link CD.
The Caenorhabditis elegans Ortholog of TDP-43 Regulates the Chromatin Localization of the Heterochromatin Protein 1 Homolog HPL-2.
Mol Cell Biol 2018 38(15)  
[ PubMed ID = 29760282 ] [ RRC reference ]

McMurchy AN, Stempor P, Gaarenstroom T, Wysolmerski B, Dong Y, Aussianikava D, Appert A, Huang N, Kolasinska-Zwierz P, Sapetschnig A, Miska EA, Ahringer J.
A team of heterochromatin factors collaborates with small RNA pathways to combat repetitive elements and germline stress.
Elife 2017 6  
[ PubMed ID = 28294943 ] [ RRC reference ]

Snyder MJ, Lau AC, Brouhard EA, Davis MB, Jiang J, Sifuentes MH, Csankovszki G.
Anchoring of Heterochromatin to the Nuclear Lamina Reinforces Dosage Compensation-Mediated Gene Repression.
PLoS Genet 2016 12(9) e1006341 
[ PubMed ID = 27690361 ] [ RRC reference ]

Kim KW, Tang NH, Andrusiak MG, Wu Z, Chisholm AD, Jin Y.
A Neuronal piRNA Pathway Inhibits Axon Regeneration in C. elegans.
Neuron 2018 97(3) 511-519.e6 
[ PubMed ID = 29395906 ] [ RRC reference ]

Lee CS, Lu T, Seydoux G.
Nanos promotes epigenetic reprograming of the germline by down-regulation of the THAP transcription factor LIN-15B.
Elife 2017 6  
[ PubMed ID = 29111977 ] [ RRC reference ]

Herbette M, Mercier MG, Michal F, Cluet D, Burny C, Yvert G, Robert VJ, Palladino F.
The C. elegans SET-2/SET1 histone H3 Lys4 (H3K4) methyltransferase preserves genome stability in the germline.
DNA Repair (Amst) 2017 57 139-150 
[ PubMed ID = 28779964 ] [ RRC reference ]

Patel T, Hobert O.
Coordinated control of terminal differentiation and restriction of cellular plasticity.
Elife 2017 6  
[ PubMed ID = 28422646 ] [ RRC reference ]

Hall SE, Beverly M, Russ C, Nusbaum C, Sengupta P.
A cellular memory of developmental history generates phenotypic diversity in C. elegans.
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[ PubMed ID = 20079644 ] [ RRC reference ]

Wagner CR, Kuervers L, Baillie DL, Yanowitz JL.
xnd-1 regulates the global recombination landscape in Caenorhabditis elegans.
Nature 2010 467(7317) 839-43 
[ PubMed ID = 20944745 ] [ RRC reference ]

Updike DL, Hachey SJ, Kreher J, Strome S.
P granules extend the nuclear pore complex environment in the C. elegans germ line.
J Cell Biol 2011 192(6) 939-48 
[ PubMed ID = 21402789 ] [ RRC reference ]

Petrella LN, Wang W, Spike CA, Rechtsteiner A, Reinke V, Strome S.
synMuv B proteins antagonize germline fate in the intestine and ensure C. elegans survival.
Development 2011 138(6) 1069-79 
[ PubMed ID = 21343362 ] [ RRC reference ]

Koester-Eiserfunke N, Fischle W.
H3K9me2/3 binding of the MBT domain protein LIN-61 is essential for Caenorhabditis elegans vulva development.
PLoS Genet 2011 7(3) e1002017 
[ PubMed ID = 21437264 ] [ RRC reference ]

Black JC, Allen A, Van Rechem C, Forbes E, Longworth M, Tschöp K, Rinehart C, Quiton J, Walsh R, Smallwood A, Dyson NJ, Whetstine JR.
Conserved antagonism between JMJD2A/KDM4A and HP1γ during cell cycle progression.
Mol Cell 2010 40(5) 736-48 
[ PubMed ID = 21145482 ] [ RRC reference ]