Mutants (Isolated)

tm1447

Allele Name

tm1447

Sequence Name

Y116A8C.12

CGC Name

arf-6

Worm Base

Allele Name

tm1447

CGC Name

arf-6

Sequence

Y116A8C.12

Phenotype

homozygous viable. Dr. M. Han: WT vulval development, slow growth and WT touch response. Dr. K. Shen: Normal HSN vulval presynaptic vesicle clusters (unc-86::SNB-1::YFP), no obvious egg laying defects. Dr. Y. Jin: no specific neuronal phenotype is found. Dr. C. Rongo: normal GLR-1::GFP localization and expression. Dr. J-L. Bessereau: no specific neuronal phenotype. Dr. G. Garriga: extra AVM/PVM in arf-1: arf-6 double mutants. Dr. O. Blacque: dye-filling normal. Dr. J. Lee: normal touch response.

Mutation site

46750/46751-49013/49014 (2263 bp deletion)

Chromosome

Putative gene structure

join(47037..47118, 47186..47289, 47380..47477, 47561..47661, 47882..47942, 49289..49370)

Map position

15.8

Balancer

Map position of balancer

Sequence of primers

ExtRev:GTCTCCGGTTGAAGCACATG,IntRev:GGTTGGACGTACCTGCAAAG,ExtFwd:CTAGGAGATTACAGGCCCAT,IntFwd:CGCAAAAACGAATGCGTAGC

Distributed lab

Depositor

Dr. S. Mitani/NBRP

References

Please submit your publication

Chen D, Yang C, Liu S, Hang W, Wang X, Chen J, Shi A.
SAC-1 ensures epithelial endocytic recycling by restricting ARF-6 activity.
J. Cell Biol. 2018 217(6) 2121-2139
[ PubMed ID = 29563216 ] [ RRC reference ]

Zhai J, Zhang L, Mojsilovic-Petrovic J, Jian X, Thomas J, Homma K, Schmitz A, Famulok M, Ichijo H, Argon Y, Randazzo PA, Kalb RG.
Inhibition of Cytohesins Protects against Genetic Models of Motor Neuron Disease.
J. Neurosci. 2015 35(24) 9088-105
[ PubMed ID = 26085633 ] [ RRC reference ]

Singhvi A, Teuliere J, Talavera K, Cordes S, Ou G, Vale RD, Prasad BC, Clark SG, Garriga G.
The Arf GAP CNT-2 regulates the apoptotic fate in C. elegans asymmetric neuroblast divisions.
Curr. Biol. 2011 21(11) 948-54
[ PubMed ID = 21596567 ] [ RRC reference ]

Gong T, Yan Y, Zhang J, Liu S, Liu H, Gao J, Zhou X, Chen J, Shi A.
PTRN-1/CAMSAP promotes CYK-1/formin-dependent actin polymerization during endocytic recycling.
EMBO J. 2018 37(9)
[ PubMed ID = 29567645 ] [ RRC reference ]

Shi A, Liu O, Koenig S, Banerjee R, Chen CC, Eimer S, Grant BD.
RAB-10-GTPase-mediated regulation of endosomal phosphatidylinositol-4,5-bisphosphate.
Proc. Natl. Acad. Sci. U.S.A. 2012 109(35) E2306-15
[ PubMed ID = 22869721 ] [ RRC reference ]

Liu H, Wang S, Hang W, Gao J, Zhang W, Cheng Z, Yang C, He J, Zhou J, Chen J, Shi A.
LET-413/Erbin acts as a RAB-5 effector to promote RAB-10 activation during endocytic recycling.
J. Cell Biol. 2018 217(1) 299-314
[ PubMed ID = 29079669 ] [ RRC reference ]

Skorobogata O, Escobar-Restrepo JM, Rocheleau CE.
An AGEF-1/Arf GTPase/AP-1 ensemble antagonizes LET-23 EGFR basolateral localization and signaling during C. elegans vulva induction.
PLoS Genet. 2014 10(10) e1004728
[ PubMed ID = 25329472 ] [ RRC reference ]

Teuliere J, Cordes S, Singhvi A, Talavera K, Garriga G.
Asymmetric neuroblast divisions producing apoptotic cells require the cytohesin GRP-1 in Caenorhabditis elegans.
Genetics 2014 198(1) 229-47
[ PubMed ID = 25053664 ] [ RRC reference ]

Gleason RJ, Akintobi AM, Grant BD, Padgett RW.
BMP signaling requires retromer-dependent recycling of the type I receptor.
Proc. Natl. Acad. Sci. U.S.A. 2014 111(7) 2578-83
[ PubMed ID = 24550286 ] [ RRC reference ]

Kimata T, Tanizawa Y, Can Y, Ikeda S, Kuhara A, Mori I.
Synaptic polarity depends on phosphatidylinositol signaling regulated by myo-inositol monophosphatase in Caenorhabditis elegans.
Genetics 2012 191(2) 509-21
[ PubMed ID = 22446320 ] [ RRC reference ]