Mutants (Isolated)

tm1447

Allele Nametm1447
Sequence NameY116A8C.12
CGC Namearf-6
Worm BaseAllele Name tm1447
CGC Name arf-6
Sequence Y116A8C.12
Phenotypehomozygous viable. Dr. M. Han: WT vulval development, slow growth and WT touch response. Dr. K. Shen: Normal HSN vulval presynaptic vesicle clusters (unc-86::SNB-1::YFP), no obvious egg laying defects. Dr. Y. Jin: no specific neuronal phenotype is found. Dr. C. Rongo: normal GLR-1::GFP localization and expression. Dr. J-L. Bessereau: no specific neuronal phenotype. Dr. G. Garriga: extra AVM/PVM in arf-1: arf-6 double mutants. Dr. O. Blacque: dye-filling normal. Dr. J. Lee: normal touch response.
Mutation site46750/46751-49013/49014 (2263 bp deletion)
ChromosomeIV
Putative gene structurejoin(47037..47118, 47186..47289, 47380..47477, 47561..47661, 47882..47942, 49289..49370)
Map position15.8
Balancer
Map position of balancer
Sequence of primersExtRev:GTCTCCGGTTGAAGCACATG,IntRev:GGTTGGACGTACCTGCAAAG,ExtFwd:CTAGGAGATTACAGGCCCAT,IntFwd:CGCAAAAACGAATGCGTAGC
Distributed lab
DepositorDr. S. Mitani/NBRP
References Please submit your publication
Chen D, Yang C, Liu S, Hang W, Wang X, Chen J, Shi A.
SAC-1 ensures epithelial endocytic recycling by restricting ARF-6 activity.
J. Cell Biol. 2018 217(6) 2121-2139 
[ PubMed ID = 29563216 ] [ RRC reference ]

Zhai J, Zhang L, Mojsilovic-Petrovic J, Jian X, Thomas J, Homma K, Schmitz A, Famulok M, Ichijo H, Argon Y, Randazzo PA, Kalb RG.
Inhibition of Cytohesins Protects against Genetic Models of Motor Neuron Disease.
J. Neurosci. 2015 35(24) 9088-105 
[ PubMed ID = 26085633 ] [ RRC reference ]

Singhvi A, Teuliere J, Talavera K, Cordes S, Ou G, Vale RD, Prasad BC, Clark SG, Garriga G.
The Arf GAP CNT-2 regulates the apoptotic fate in C. elegans asymmetric neuroblast divisions.
Curr. Biol. 2011 21(11) 948-54 
[ PubMed ID = 21596567 ] [ RRC reference ]

Gong T, Yan Y, Zhang J, Liu S, Liu H, Gao J, Zhou X, Chen J, Shi A.
PTRN-1/CAMSAP promotes CYK-1/formin-dependent actin polymerization during endocytic recycling.
EMBO J. 2018 37(9)  
[ PubMed ID = 29567645 ] [ RRC reference ]

Shi A, Liu O, Koenig S, Banerjee R, Chen CC, Eimer S, Grant BD.
RAB-10-GTPase-mediated regulation of endosomal phosphatidylinositol-4,5-bisphosphate.
Proc. Natl. Acad. Sci. U.S.A. 2012 109(35) E2306-15 
[ PubMed ID = 22869721 ] [ RRC reference ]

Liu H, Wang S, Hang W, Gao J, Zhang W, Cheng Z, Yang C, He J, Zhou J, Chen J, Shi A.
LET-413/Erbin acts as a RAB-5 effector to promote RAB-10 activation during endocytic recycling.
J. Cell Biol. 2018 217(1) 299-314 
[ PubMed ID = 29079669 ] [ RRC reference ]

Skorobogata O, Escobar-Restrepo JM, Rocheleau CE.
An AGEF-1/Arf GTPase/AP-1 ensemble antagonizes LET-23 EGFR basolateral localization and signaling during C. elegans vulva induction.
PLoS Genet. 2014 10(10) e1004728 
[ PubMed ID = 25329472 ] [ RRC reference ]

Teuliere J, Cordes S, Singhvi A, Talavera K, Garriga G.
Asymmetric neuroblast divisions producing apoptotic cells require the cytohesin GRP-1 in Caenorhabditis elegans.
Genetics 2014 198(1) 229-47 
[ PubMed ID = 25053664 ] [ RRC reference ]

Gleason RJ, Akintobi AM, Grant BD, Padgett RW.
BMP signaling requires retromer-dependent recycling of the type I receptor.
Proc. Natl. Acad. Sci. U.S.A. 2014 111(7) 2578-83 
[ PubMed ID = 24550286 ] [ RRC reference ]

Kimata T, Tanizawa Y, Can Y, Ikeda S, Kuhara A, Mori I.
Synaptic polarity depends on phosphatidylinositol signaling regulated by myo-inositol monophosphatase in Caenorhabditis elegans.
Genetics 2012 191(2) 509-21 
[ PubMed ID = 22446320 ] [ RRC reference ]