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Y. Ogihara (Kihara Inst Biol Res, Yokohama City Univ;
ogihara@yokohama.cu.ac.jp)
Functional plastomics in wheat: Monitoring of whole plastome gene
expressions in the wheat life cycle as revealed by the plastid DNA
microarray method
Plastids develop from proplastids to differentiated organella
such as chloroplasts, etioplasts, chromoplasts, leucoplasts and
amyloplasts. All plastids contain circular DNA with long inverted
repeats that separate the rest of the molecule into small and large
single copy regions. Recently, the complete nucleotide sequence of
chloroplast DNA for Chinese Spring wheat (134,540 bp) has been
determined. The 108 plastid genes, so far deduced, as well as 16
light-responsive and ten cold-inducible genes were spotted onto the
microarray in order to investigate profiling of gene expressions of
whole plastid genes in the wheat life cycle. RNAs were extracted from
13 different tissues: seedlings grown under light and in the dark.,
roots, fourth leaves at the heading stage, flag leaves at the heading
stage, young spikelets, spikes at the heading stage, spikes at the
flowering stage, seeds at 5,20, 40, 50 days after pollination (DAP),
and embryo. The cDNAs were labelled by incorporation of fluorolink
Cy5-dUTP, and hybridized to the spotted genes. The hybridization
signals of each gene in each tissue were measured, and compared. Each
tissue showed representative hybridization patterns, displaying
different gene expression patterns among tissues except the patterns
between young seedling grown under light and fourth leaves at the
heading stage.
K. Tsunewaki*, K. Ichikawa, I. Oda and K. Morinaga
(Dep Bioscience, Fukui Pref Univ; *tunewaki@fpu. ac. jp)
Genetic analysis of three kinds of chlorophyll abnormality
appeared in tetiraploid offspring of a pentaploid wheat hybrid
Three chlorophyll abnormalities, striato-virescence, delayed
virescence and albino, appeared in tetraploid offspring of the
crosses, T. durum cv. Langdon x [ (T. aestivum
cv. Jones Fife x T. dicoccum cv. Vernal) x T.
dicoccum cv. Vernal3], where Vernal3
means three backcrosses with Vernal as the recurrent parent.
Striato-virescence is the appearance of albinotic stripes in leaves
of the tillers formed only in winter. Delayed virescence is change of
normally green seedlings to albinotic ones in mid-winter to early
spring, with slow recovery of green color later. Albino is
chlorophyll-deficiency of germinating seedlings. These three
abnormalities were subjected to the aneuploid analysis using the
disomic substitution series of Langdon durum. The analyses suggested
that all three abnormalities are controlled by two recessive alleles;
striato-virescence by sv1 on chromosome 3A and sv2 on
2A, delayed virescence by dv1 on 2A and dv2 on 2B, and
albino by abn1 on 2A and abn2 on 2B. T. aestivum
cv. Chinese Spring carries their normal homoeoalleles,
Sv3, Dv3 and Abn3 all on 2D chromosome.
4. Poster session
T. Ibuki, J. Suzuki, Y. Kowyama and K Kakeda* (Fac
Bioresour, Mie Univ; *kakeda@bio. mie- u.ac.jp)
Search for self-incompatibility related gene transcripts in
Hordeum bulbosum
A. Meguro1, S. Takumi2, Y.
Ogihara3 and K. Murai1* (1Dept
Biosci,Fukui Pref Univ, 2Fac Agri, Kobe Univ,
3Kihara Inst Biol Res, Yokohama City Univ;
*murai@fpu.ac.jp)
Cloning and characterization of wheat AGAMOU homologous gene,
WAG
T. Kawahara1, S. Taketa2 and K.
Murai1* (1Dept Biosci, Fukui Pref Univ,
2Fac Agri, Kagawa Univ; *murai@fpu.ac.jp)
Development of RAPD markers useful for identifying wheat-barley 5H
chromosome recombinant lines
H. Kato and K Murai* (Dept Biosci, Fukui Pref Univ;
*murai@fpu.ac.jp)
Detection of DNA markers linked to Vrn-A1 gene by bulked
segregant analysis.
T. Yoshii and K. Murai* (Dept Biosci, Fukui Pref Univ;
*murai@fpu.ac.jp)
Cloning of novel genes expressed in wheat young spike
S. Hirosawa1, N. Mori1'*, S.
Takumi1, T. Kawahara2 and C.
Nakamura1 (1Fac Agr, Kobe Univ,
2Fac Agr, Kyoto Univ)
AFLP analysis for the phylogeny and evolution of common
wheat
H. Tanaka1, N. Mori*, S. Takumi1, T.
Kawahara2 and C. Nakamura1 (1Fac
Agr, Kobe Univ, 2Fac Agr, Kyoto Univ)
Allelic diversity at chloroplast microsatellite loci among diploid
species of Aegilops and Triticum
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