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Inter-relationship among parents
F2 seedlings of crosses of HD 2135 with four other
parents, HD 2160, HD 2189, HD 2285 and Vaishali, when analysed with
21(9G5), 21A-2(75G5) and 117A(36G2) did not segregate for
susceptibility (Table 3). All the seedlings
showed; or 0; reaction and indicated that a common factor(s)
provided resistance to above pathotypes. However, segregation in the
ratio 61R: 3S in cross HD 2135 x Vaishali to 40-1(62G29-1) showed the
involvement of two dominant and one recessive factors for
resistance.
With pathotypes 21(9G5), 21A-2(75G5) and 117A(36G2), F2
seedlings of crosses HD 2160 with HD 2189, HD 2285 and Vaishali
showed only resistant seedlings due to common gene(s) for resistance
in them. F2 seedlings of crosses HD 2160 x HD 2189 and HD
2160 x HD 2285 also showed resistance to 40-1(62G29-1), but
segregated in 61R: 1S ratio in HD 2160 x Vaishali. The above
segregation explains the presence of two dominant and one recessive
gene providing resistance to above pathotype.
HD 2189, when crossed with HD 2285 and Vaishali, F2
population showed resistance to all pathotypes. Also, F2
seedlings of cross HD 2285 x Vaishali did not segregate for
susceptibility and showed a common factor providing resistance to
21(9G5), 21A-2(75G5), 117A(36G2) and 40-1(62G29-1).
Test of allelism with isogenic lines
Sharma (1990) postulated genes Sr2, Sr11 and Sr30 in HD
2189; and genes Sr2, Sr5, Sr11, Sr17 and Sr30 in HD
2285 with Australian pathotypes of stem rust. Therefore, HD 2189 and
HD 2285 were crossed with two isogenic wheat lines Sr11 and
Sr30. The F2 seedling analysis of above crosses did not show
segregation for susceptibility to pathotypes 21(9G5) and 117A(36G2)
and confirmed the presence of Sr11 and Sr30 in HD 2189
and HD 2285. Crosses of these parents with Sr2 and Sr17
were not made since Sr2 expresses in adult plants and
Sr17 does not provide resistance to Indian pathotypes of stem
rust. Also crosses of parents with Sr5 could not be made due
to non synchronous flowering.
An adult plant resistance gene Sr2 was identified in HD 2135,
HD 2189, HD 2285 and Vaishali based on mottling effect in the
seedlings (McIntosh 1992). Sr2 was initially derived from
'Yaroslav' a tetraploid wheat by McFadden (1930). Inheritance of
resistance in CIMMYT semidwarfs is associated with Sr2 gene
complex derived from variety 'Newthatch'(Rajaram et al. 1988).
Through CIMMYT wheats, Sr2 has gone to many developing
countries including India. Sr2 was also identified in many
other Indian wheats (Bahadur et al.1993).
Sharma (1990) identified Sr30 in 29 wheat varieties with
Australian pathotypes of stem rust. Gene Sr11 was identified
in cvs. HD 2189 and HD 2285 in the present study. Gandhi (1967)
reported an additional gene along with Sr11 in E 581. Sr11
also conferred resistance in two sister selections of Kalyansona-
Siete cerros and Indus 66 (Mclntosh 1988) and in 15 other wheats of
Indian origin (Bahadur et al. 1993). The other genes identified in HD
2135, HD 2160 and Vaishali require further confirmation
through test of allelism.
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