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HD 2189
The interaction of all test pathotypes was; on F₁ of cross HD 2189 x AL, similar to HD 2189. Out of 443 F2 seedlings, 435 segregated for resistance and 8 susceptibility deriving the ratio 63R: 1S to 21 (9G5). Seedlings also segregated in the ratio 63R: 1S in reciprocal cross and the F3 families showed the pattern 37R: 26Seg: 1S. F₂ seedlings of both direct and reciprocal crosses showed 63R: 1S ratio to pathotype 21A-2(75G5) and 117A (36 G 2). The above ratio was further confirmed by F₃ families, which segregated in 37R: 26Seg: 1S. To race 40-1(62G29-1), F₂ seedling segregated in the ratio 15R: 1S both in direct and reciprocal crosses. The above ratio was further confirmed by the analysis of F₃ families, which segregated in 7R: 8Seg: 1S. The studies revealed the presence of three dominant independent genes for resistance to pathotypes 21(9G5), 21A-2(75G5) and 117A(36G2) and two dominant independent genes for resistance to 40-1(62G29-1).

HD 2285
F₁ of cross HD 2285 x AL showed ;1 reaction to all the test pathotypes except 40-1(62G29-1). Out of 269 seedlings, 265 segregated for resistance and 4 for susceptibility, deriving the ratio 63R: 1S to 21(9G5). The seedlings of direct and reciprocal crosses also segregated in above ratio to 21A-2(75G5). Segregation of F₃ families in 37R: 26Seg: 1S further confirmed the above segregation pattern. To 40-1(62G29-1), seedlings in F₂ segregated in the ratio 1R: 15S and F₃ families in 1R: 8 Seg: 7S indicating the presence of 2 recessive complementary genes in HD 2285. Segregation of seedlings of both direct and reciprocal cross occurred in 15R: 1S ratio to 117A(36G2), which was confirmed by the grouping of F₃ families in 7R: 8Seg: 1S. The above analysis showed the presence of 3 dominant independent genes for resistance to pathotypes 21(9G5) and 21A- 2(75G5); two dominant independent genes to 117A(36G2); and two recessive complementary genes to 40-1(62G29-1).

Vaishali
In cross Vaishali x AL, the interaction in F1 was 0,; and 1 to 21(9G5), 21A2(75G5) and 117A(36G2) respectively. The host pathogen interaction was ;1 to 40-1(62G29-1). Two hundred ninety nine: seedlings of F₂ segregated in 297 resistant and 2 susceptible deriving the ratio 255R: 1S to 21(9G5). The above ratio was also obtained with pathotype 21A-2(75G5), which was also confirmed by segregation pattern of the reciprocal crosses to above two pathotypes. To pathotypes 40-1(62G29-1) and 117A(36G2), F₂ seedlings also segregated in the ratio 15R: 1S and 63R: 1S respectively. F₃ families of the above cross also showed the segregation pattern 37R: 26Seg: 1S to pathotype 117A(36G2). F₂ and F₃ analysis confirmed the presence of four dominant independent genes for resistance to pathotype 21(9G5) and 21A-2(75G5); three dominant independent genes for resistance to 117A(36G2) and two dominant independent genes for resistance to 40-1(62G29) in Vaishali.

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