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F2 segregations of chromosome complements and -amylase isozyme bands are shown in Table 4, from which recombination values between markers are estimated.

alpha-Amy-B1 - alpha-Amy-B5 　

less than 1 %

alpha-Amy-B1 - alpha-Amy-B4

22.3 plus or minus 4.6%

centromere - alpha-Amy-B1　

13.2 plus or minus 3.5%

centromere - alpha-Amy-B5

2.1 plus or minus 1.1%

centromere - alpha-Amy-B4

26.1 plus or minus 5.0%

There are considerable discrepancies among the recombination values calculated, which seem due to too small population size, especially to obtain double recessive segregants from F1 hybrid in a repulsion phase. That is, the recombination values between alpha-Amy-B1 and alpha-Amy-B4, and also between centromere and alpha-Amy-B5 seem to be considerably underestimated, because some of crossover types were deficient. Taking these conditions into consideration we drew the tentative map as shown in Fig.3-c.


References

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Endo TR and Mukai Y (1988) Chromosome mapping ofa speltoid suppression gene of Triticum aestivum L. based on partial deletions in the Ion. arm of chromosome 5A. Jpn J Genet 63: 501-505.

Gale MD (1983) Alpha-amylase genes in wheat. Proc 3rd Intern Symp Preharv Sprout Cereals: 105-110.

Hart GE, Gale MD and McIntosh (1993) Linkage maps of Triticum aestivum (Hexaploid Wheat, 2n=42, Genomes A. B & D) and T. tauschii (2n=14, Genome D). Pro.. Genome Map. Wheat and Relat. Species (Proc 3rd Public Workshop Intern Triticeae Map Init).

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Nishikawa K (1991) Chromosome mapping by use of aneuploids in wheat. Wheat Inf Serv: 60-63.

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Tsujimoto H and Noda K (1990) Deletion mapping by gametocidal genes in common wheat: Position of speltoid suppressor (Q) and beta-amylase (beta-Amy-A2) gene on chromosome 5A. Genome 33: 850-853.

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