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Wheat Information
Service
Number 77: 39-45 (1993)
Telocentric
mapping of alpha-amylase loci in wheat
K. Nishikawa1, T. Ban2 and Y. Furuta
Faculty of Agriculture, Gifu University, 101 Yanagido, Gifu
501-11, Japan
Summary
With the improved technique of electrophoresis the precise
genetical analyses of alpha-amylase isozymes were realized with the
following results;
Aka, a Japanese local variety is different from Chinese Spring in
Band 20 coded by alpha-Amy-A1, which was located on the long
arm apart from the centromere of chromosome 6A with recombination
value of 3.7 plus or minus 0.01 (%). In comparison with Chi inese
Spring a common wheat cultivar, Jones Fife have Band 15 instead of
Band 18, which is coded by the alleles of alpha-Amy-B1, and
Band 1 which is deficient in Chinese Spring and coded by
alpha-Amy-B3. As the results of three point analysis,
centromere -alpha-Amy-B3 -alpha-Amy-B1 were mapped in
this order with recombination values, 5.5 plus or minus 1.7(%) and
9.3 plus or minus 2.2(%), respectively. Several isozyme bands vary
between georgicum and durum LD 222, and the telocentric
mapping showed that the map distances between centromere
-alpha-Amy-B1(alpha-Amy-B5) -alpha-Amy-B4
were tentatively determined 13.2 plus or minus 3.5(%) and 22.3
plus or minus 4.6(%).
Introduction
With a limited number of morphological markers, the linkage map
in wheat is still insufficient in comparison with some other crops
such as corn, rice, barley and so on. Various kinds of aneuploid
series are available in wheat, and telocentric mapping is
distinguished for being able to determine the centromere position,
and results in the reduced map distance (Nishikawa 1991). Hart et al.
(1993) summarized linkage map of hexaploid wheat and T.
tauschii. On the other hand, deletion mapping (Endo and Mukai,
1988; Tsujimoto and Noda, 1990), and in situ hybridization
mapping (Mukai, 1991) are now developing.
It was in 1981 that Nishikawa et al. first reported genetic analyses
of alpha-amylase loci, and thereafter with improved electrophoresis
technique more precise analyses could be realized, which we will
report in this paper.
Present
address:
1 Aichi Sangyo University, Okamachi 12-5, Okazaki 444,
Japan.
2 Kyushu National Agricultural Experiment Station, lzumi
496, Chikugoshi 833, Japan
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