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Wheat Information Service
Number 77: 39-45 (1993)

Telocentric mapping of alpha-amylase loci in wheat

K. Nishikawa1, T. Ban2 and Y. Furuta

Faculty of Agriculture, Gifu University, 101 Yanagido, Gifu 501-11, Japan


With the improved technique of electrophoresis the precise genetical analyses of alpha-amylase isozymes were realized with the following results;

Aka, a Japanese local variety is different from Chinese Spring in Band 20 coded by alpha-Amy-A1, which was located on the long arm apart from the centromere of chromosome 6A with recombination value of 3.7 plus or minus 0.01 (%). In comparison with Chi inese Spring a common wheat cultivar, Jones Fife have Band 15 instead of Band 18, which is coded by the alleles of alpha-Amy-B1, and Band 1 which is deficient in Chinese Spring and coded by alpha-Amy-B3. As the results of three point analysis, centromere -alpha-Amy-B3 -alpha-Amy-B1 were mapped in this order with recombination values, 5.5 plus or minus 1.7(%) and 9.3 plus or minus 2.2(%), respectively. Several isozyme bands vary between georgicum and durum LD 222, and the telocentric mapping showed that the map distances between centromere -alpha-Amy-B1(alpha-Amy-B5) -alpha-Amy-B4 were tentatively determined 13.2 plus or minus 3.5(%) and 22.3 plus or minus 4.6(%).


With a limited number of morphological markers, the linkage map in wheat is still insufficient in comparison with some other crops such as corn, rice, barley and so on. Various kinds of aneuploid series are available in wheat, and telocentric mapping is distinguished for being able to determine the centromere position, and results in the reduced map distance (Nishikawa 1991). Hart et al. (1993) summarized linkage map of hexaploid wheat and T. tauschii. On the other hand, deletion mapping (Endo and Mukai, 1988; Tsujimoto and Noda, 1990), and in situ hybridization mapping (Mukai, 1991) are now developing.

It was in 1981 that Nishikawa et al. first reported genetic analyses of alpha-amylase loci, and thereafter with improved electrophoresis technique more precise analyses could be realized, which we will report in this paper.

Present address:
1 Aichi Sangyo University, Okamachi 12-5, Okazaki 444, Japan.
2 Kyushu National Agricultural Experiment Station, lzumi 496, Chikugoshi 833, Japan


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