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Wheat Information Service
Number 77: 25-28 (1993)

Monosomic analysis for total and sterile floret number per spike in the multispikelet line 10-A of common wheat

Y. L. Zheng1, C. Yen2 and J. L. Yang2

1
Department of Agronomy, Sichuan Agricultural University, Yaan City, Sichuan, 625014, P. R. China

2 Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan City, Sichuan, 611830, P. R. China


Summary

Chromosome location of the genes for total and sterile floret number per spike in the common wheat was carried out by using multispikelet line 10-A and two sets of monosomic lines, Chinese Spring and Abbondanza. The large number of total florets per spike of 10-A was controlled by the chromosomes 5A, 7A, 1 B, 2B, 2D and 6D, but the genes on the chromosomes 1 B, 2B, 2D and 6D controlled mainly the high number of sterile florets per spike. The useful value of 10-A was discussed in wheat breeding of increasing grain number per spike as main objective.


Introduction

Yen et al. (1993) selected a common wheat, multispikelet line 10-A by using the genetic resources for large number of spikelets per spike from rye. The large number of florets per spike results entirely from increasing spikelet number per spike, and so this kind of germplasm undoubtedly is very useful ideotype of increasing the grain number per spike in wheat breeding (Zheng et al, 1992). Unfortunately, the number of the sterile florets per spike of "10-A" is much higher than that of the popular wheat varieties, resulting in giving rise to the less grain number of "10-A". This study aimed to locate the genes governing the total and sterile floret number per spike of 10-A on certain chromosome.


Materials and methods

The tested line 10-A was selected from the offsprings of backcrossing Triticale (8x) with common wheat by Yen et al. (1993). Two sets of monosomic lines, Chinese Spring and Abbondanza, were used in the monosomic analysis. From each monosomic line, two or three monosomic plants were pollinated with 10-A. Three monosomic and at least one disomic plants were mitotically selected from each F1 hybrid. The selected F1 plants were observed with respect to the total and sterile floret number per spike. Of the each set, 21 F2 populations derived from monosomic F1 hybrid for different chromosomes and one euploid F2 population derived from disomic F1 plants were included, and these two characters were investigated on 160 plants of each F2 population.

In the statistic analysis of data, the number of total or sterile florets per spike was maximum selected from the 5-8 observed spikes of each sample plant. The significance of differences between the mean values were detected by t-test. F-test was adopted to detect the significance of the differences between the variances.

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