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Wheat Information
Service
Number 75: 63-67 (1992)
Storage
effect on seedling characters of monosomics in
wheat
Dalmir Singh
Division of Genetics, Indian Agricultural Research Institute, New
Delhi 110012, India
Summary
The study of the storage effect on the gene products (controlled by
individual chromosomes) stored in the embryo and endosperm which are
responsible for the initial development of plant parts in monosomic
and disomic lines of wheat variety Pb. C591 has revealed the facts
listed below.
1. Coleoptile length is promoted by at least nine chromosomes (1B,
2B, 3A, 4A, 5A, 6A, 6B, 7A, and 7D), while chromosome 7B was found to
inhibit the coleoptile length. It is observed that chromosomes 1B, 4A
and 5A carry genes for major effect to promote the trait.
2. Chromosomes 1B, 4A, 5A and 6A carry genes for seedling height
promoter, while chromosome 7B inhibits the seedling height.
3. Root length is affected by at least ten chromosomes, 1A, 1B, 4A,
4B, 4D, 5A, 5D, 6D, 7A and 7D respectively.
Introduction
The development of initial plant parts like coleoptile, root and
shoot are as important as the other traits related directly to wheat
yield. These traits are extremely important for initial development
and proper stand of the plant. It is therefore very necessary to
study the effect of genetic factors on the development of coleoptile,
roots and shoots in hexaploid wheat. These developmental stages are
greatly influenced by the stored material present in the embryo and
endosperm which are under the control of genetic factors. The
environmental conditions like temperature and moisture also affect
these processes to some extent. It was therefore thought necessary to
study the storage effect on these developmental processes in
monosomic lines of variety Pb. C591.
Materials and methods
To study the effect of storage on individual chromosomes of variety
Pb. C591, all the monosomic lines except monosomic 5B were used. The
monosomic plants were identified cytologically at first meiotic
metaphase continuously for four years. The seeds were harvested from
all the cytologically identified monosomic and disomic plants
separately and were stored every year at 12 + or -1oC
inside the refrigerator. From each of the monosomic and disomic line,
200 seeds were germinated in separate petridishes (four petridishes
in each case) at 25 + or - 1oC.
After 8 or 9 days
of imbibition, data were recorded on coleoptile length, seedling
height and root length.
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