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Wheat Information Service
Number 75: 63-67 (1992)

Storage effect on seedling characters of monosomics in wheat

Dalmir Singh

Division of Genetics, Indian Agricultural Research Institute, New Delhi 110012, India


The study of the storage effect on the gene products (controlled by individual chromosomes) stored in the embryo and endosperm which are responsible for the initial development of plant parts in monosomic and disomic lines of wheat variety Pb. C591 has revealed the facts listed below.

1. Coleoptile length is promoted by at least nine chromosomes (1B, 2B, 3A, 4A, 5A, 6A, 6B, 7A, and 7D), while chromosome 7B was found to inhibit the coleoptile length. It is observed that chromosomes 1B, 4A and 5A carry genes for major effect to promote the trait.

2. Chromosomes 1B, 4A, 5A and 6A carry genes for seedling height promoter, while chromosome 7B inhibits the seedling height.

3. Root length is affected by at least ten chromosomes, 1A, 1B, 4A, 4B, 4D, 5A, 5D, 6D, 7A and 7D respectively.


The development of initial plant parts like coleoptile, root and shoot are as important as the other traits related directly to wheat yield. These traits are extremely important for initial development and proper stand of the plant. It is therefore very necessary to study the effect of genetic factors on the development of coleoptile, roots and shoots in hexaploid wheat. These developmental stages are greatly influenced by the stored material present in the embryo and endosperm which are under the control of genetic factors. The environmental conditions like temperature and moisture also affect these processes to some extent. It was therefore thought necessary to study the storage effect on these developmental processes in monosomic lines of variety Pb. C591.

Materials and methods

To study the effect of storage on individual chromosomes of variety Pb. C591, all the monosomic lines except monosomic 5B were used. The monosomic plants were identified cytologically at first meiotic metaphase continuously for four years. The seeds were harvested from all the cytologically identified monosomic and disomic plants separately and were stored every year at 12 + or -1oC inside the refrigerator. From each of the monosomic and disomic line, 200 seeds were germinated in separate petridishes (four petridishes in each case) at 25 + or - 1
oC. After 8 or 9 days of imbibition, data were recorded on coleoptile length, seedling height and root length.


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