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Most of the anthers were found dorsally attached to the mature grains in cultivars DWL 5023, WL 1562, WL 711, WG 377 and TL 419 (Fig. 1). Most likely, these anthers were not fully mature to attain normal versatile attachment. These did not dehisce probably due to incomplete maturation or water imbalance in the anther/anther walls, thus affecting pollination and fertilization in these cultivars.

The number of grains produced could be a useful indicator of the number of flowers fertilized as well as conditions required for setting and filling of grains. However, in this study the number of grains was not indicative of total grain yield, as in cultivars WL 711, WG 357, KSML 3 and WL 410, the grain filling was incomplete. Even in dried ears, the grains were only at half seed stage and appeared to be dry. The inhibited translocation did not appear to be the only reason for this effect because in that case, shrivelled but complete grains were likely to be produced. Thus, in addition to interrupted translocation, the grain development was also incomplete. Thus cultivars TL 419, HD 2009, C 306 and WG 377 appeared to be relatively tolerant to Mn stress implying that process of fertilization and grain development were not much harmed by Mn stress. This would indicate preferential utilization of Mn or capacity of the cultivars to manage with limited supply of Mn.

Average Wt. of 100 grains (Translocation of assimilates to the developing grains)

The average 100 grain wt. of cultivars increased significantly with Mn application, but the differences among 5, 10 and 20 mg Mn/kg soil, were non-significant except C 306 at 20 mg Mn/kg rate (Table 5). Under Mn stress condition, the maximum 100 grain wt. was in cultivars WG 357 (low susceptible) and WL 410 (moderately susceptible).

Thus in tolerant cultivars, the number of grains formed were more (showing efficiency of fertilization) but the grain weight was not correspondingly increased. This indicated lesser availability of photosynthates than the sink requirement in these cultivars. In less susceptible cultivars (WG 357, WL 410) the number of grains formed was low, the limited amount of photosynthates distributed among lesser grains resulted in increase in grain weight.

Wardlaw (1968) and Bremner (1972) emphasized on the ability of the individual grain to grow and precipitate carbohydrates rather than the translocation as the major determinent of seed growth. Asana (1974) advocated that during the early stages of grain ontogeny when the assimilates were not limiting, some inherent factor(s) must be controlling the growth of grains which differed within same ear as well as variety. Dua & Bhardwaj (1979) have shown that variations among varieties with regard to 100 grain wt were traceable to endogenous auxin and cytokinin production of the variety vis.a vis that of the ear. It is imperative to study the varietal differences in content of endogenous growth regulators in the flag leaf, flowers/ears.



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