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Grain yield

Mn stress reduced the grain yield to nil in cultivars DWL 5023 and WL 1562 and very poor in cultivars WL 711, WG 377, KSML 3 and WG 357 (Table 1). Grain filling was not complete in WG 377. As the differences among these cultivars were non-significant these were susceptible to Mn deficiency. Grain yield response increased successively and significantly up to 20 mg Mn/kg rate in DWL 5023, upto 10 mg Mn/kg in KSML 3, WL 410 and TL 419 and upto 5 mg Mn/kg in WL 711, WG 377, WG 357, HD 2009 and C 306.

Straw yield

In control pots, the straw yield of DWL 5023, WL 711 and WL 1562 was very poor as compared to other cultivars (Table 1). The yield of TL 419 and HD 2009 increased significantly upto 5 mg Mn/kg-1 but of rest of cultivars upto 10 mg Mn kg-1.

Harvest index

Harvest index was considerably reduced in highly and moderately susceptible cultivars which had higher index than tolerant cultivars when adequate Mn fertilization was supplied (Table 2). Optimum harvest index in most cultivars was obtained with 5-10 mg Mn kg-1 except in DWL 5023 where the response was upto 20 mg Mn kg-1 indicating a higher requirement of Mn for reaching the potential yield.

The previous studies also indicated that tolerant cultivars were not necessarily the highest yielders when adequate supply of plant nutrients was available (Wegrzyn et al. 1980).

Increased assimilate production and reduction in assimilate wastage

The cultivars which are able to reduce assimilate wastage and have higher efficiencies to allocate assimilates are likely to show higher harvest indices. Rate of photosynthesis as measured by POE has already been reported (Kaur et al. personal communication). The effect on Mn deficiency on the following parameters was studied:

Number of infertile tillers

The number of infertile tillers is indicative of assimilate wastage, its magnitude lowering the harvest index of the plant. In highly susceptible cultivars, the 20 mg Mn kg-1 application highly increased the number of infertile tillers (Table 2). Thus the applied Mn although contributed to increased assimilate production but also resulted in its wastage because of the failure of reproductive mechanism in the infertile tillers. On the contrary, the tolerant cultivars had more infertile tillers under Mn stress and its alliviation produced more fertile tillers, thus increasing the ratio of fertile/infertile tillers resulting in higher harvest index.

Total number of tillers

The number of tillers formed were significantly less in Mn Stressed plants of DWL 5023, WL 1562 and WL 711 (Table 3) possibly reducing assimilate production. Application of even 5 mg Mn kg-1 significantly increased the tiller number to the level of more tolerant cultivars. The tiller number had a significant positive relationship in control plants with straw yield (r = 0.90**) and grain yield (r = 0.72**). The increased tiller number obviously increased assimilate production capacity of the plant.

Average Height of tillers

Tiller height increased significantly with Mn application especially 10 mg Mn kg-1 rate. The tiller height was minimum in DWL 5023 especially under Mn stress. It was significantly related to grain yield in control and 5 mg Mn kg-1 supplied plants (r = 0.81** and 0.72** respectively) and straw yield in control and 10 mg Mn kg-1 supplied plants (r = 0.71** and 0.84** respectively).



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