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Usually, a large number of the plant traits are modified as a result of interaction between a Triticum genome and alien cytoplasms. The magnitude of cytoplsmic effects depends on the nuclear genotype as well as the environmental conditions in which plants are grown. Possibly, several plasma-sensitive nuclear genes in the Triticum genome may produce observed phenotypic alterations, and a similar number of the critical nuclear genes from the respective cytoplasm donor species control the effects on the expression of the plasma-sensitive genes. A majority of the critical nuclear genes remain closely linked because of their location on an alien chromosome segment which may not (or may less often) pair with a homeologous wheat chromosome segment. For example, the 43-chromosome (monosomic-addition) wheat plants with T. boeoticum cytoplasm and a critical T. boeoticum chromosome have near-normal fertility and plant vigor (MAAN, unpublished). They produce a few plump and viable seeds having embryos with the critical T. boeoticum chromosome, but a majority of the seeds lack the critical chromosome and are shrivelled. Some of the shrivelled seeds are viable and they produce male-sterile plants of greatly reduced vigor. After one or two additional backcrosses, only inviable seeds are produced. Similar results are obtained from the 43-chromosome (monosomic-addition) wheat plants with a critical Secale cereale chromosome and S. cereale cytoplasm (MAAN and LUCKEN, 1972). The T. boeoticum and S. cereale cytoplasms induce male sterility and reduction of plant vigor in wheat plants. The plants with T. boeoticum cytoplasm have sterile pollen grains and smaller anthers than those with S. cereale cytoplasm. Therefore. T. boeoticum and S. cereale cytoplasms affect different plasma-sensitive genes of T. aestivum. Also, nuclear genes on the critical T. boeoticum chromosome do not control effects due to S. cereale cytoplasm and genes on critical S. cereale chromosome do not control effects due to T. boeoticum cytoplasm. Therefore, the species-specific plasma-sensitive genes of T. aestivum which interact with T. boeoticum cytoplasm may be different from those which interact with S. cereale cytoplasm, and specific nuclear genes on critical T. boeoticum or S. cereale chromosomes control effects due to these cytoplasms.

Nucleo-cytoplasmic interactions involving Triticum genomes and Aegilops cytoplasms indicate that several species-specific plasma-senstive genes from the cytoplasm donor species control these interactions. Since interspecific hybrid sterility and loss of plant vigor of hybrid progenies are among the major genetic isolating mechanisms in related plant species, the interspecific nucleocytoplasmic differentiation may be intimately involved in the evolutionary processes separating species. Therefore, interspecific and intergeneric nucleocytoplasmic interactions may indicate relative genetic relationships among the species of Triticum and Aegilops. The inviability of T. aestivum in the cytoplasms of T. boeoticum or S. cereale in the absence of specific nuclear genes from the respective cytoplasm donors and specificity of the cirtical nuclear genes controlling cytoplasmic effects indicates a remote genetic distance between T. boeoticum and S. cereale and between each of these two species and T. aestivum.


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