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Culm length was measured from the crown to the base of
the spike of the longest tiller of each plant and length of
the spike was measured on the same tiller. Averages for
F1, F2 and Sava monosomics were
determined for both characters by means of frequency
distribution and the averages for each of the monosomic
lines, or monosomic progenies were compared to normal
F1, F2 and Sava using the Student's
test (t test).
Results and Discussion
Culm length : Variety Sava had the average culm
length about 65 cm (Tab. 1). The
differences in that character between plants grown in
glasshouse or in field, even in different years, were not
high but the differences in culm length between Sava and
F1 or F2 disomics were highly
significant. None of the F1 monosomics or
F2 populations from F1 monosomics were
significantly longer than normal F1 or
F2 plants. The lines involving chromosomes
belonging to group 2 and 4 (except 4A) and 3B chromosomes
had average culm length smaller than corresponding disomics
and the differences were highly significant. The differences
in culm length between Sava monosomics and normal Sava were
significant for most of the monosomics (Tab.
1. Graph. 1).
Length of the Spike : Variety Sava had spike length
about 9 cm and spike length means of normal F1
and F2 were slightly smaller. Significant
differences were found between spike length means of the
F1 monosomics and normal F1 for
chromosomes 4B (shorter) and 5A and 6D(longer). Besides 5A
and 5D chromosomes in F2 population all three
members of homoeologous group 1 and chromosomes 6B and 7B
had significantly longer spikes and only for 4B and 3D
shorter (Tab. 2). Spike length
means of several Sava monosomics are significantly smaller
than normal Sava and none of them significantly longer
(Graph . 2).
Each character is controlled by the genes located on several
chromosomes and for different character different
chromosomes are critical ones.
The effects observed for critical chromosomes were mainly in
decreasing the mean values. The mean values for critical
lines are mainly the results of the effects due to the
chromosome missing.
According to LARSON (1959) the F2 monosomic
analyses of quantitative characters is the most effective in
identifying critical chromosomes of the monosomic donor
parent. In the present study variety Chinese Spring was the
monosomic donor parent and that might be the reason that the
results obtained were rather parallel to the results
presented by SEARS (1954).
Finally, the use of F1, F2 monosomic
analyses is not the best way for the chromosomal location of
the quantitative genes and some other methods should be
used.
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