| Monosomic analyses of the culm length and length of
the spike in wheat cultivar Sava S. PETROVIC Department of Genetics and Plant Breeding, Faculty of Agriculture, Novi Sad, Yugoslavia Introduction The use of monosomic analyses for the chromosomal location of the qualitative genes is as precise as the use of substitution lines. The use of monosomic analyses for the chromosomal location of the quantitative genes is not so precise since it is not possible to distinguish the effects due to chromosome dosage from the effects due to allelic differences (LAW and WORLAND, 1972). The genes controlling quantitative characters have been located in specific chromosomes using substitution lines precisely (KUSPIRA and UNRAU, 1957; HERMSEN, 1963 ; LAW, 1967; and others). However, this method requires many backcrosses besides other disadvantages. Because the monosomic analyses is somewhat easier many authors have used it (ALLAN and VOGEL, 1963, 1964 ; SHARMA and BHOWAL, 1973 ; MAYSTRENKO and CHERNYKH, 1973 ; BAIER et al., 1974 ; BARAS and KOSNER, 1974 and others). The results obtained using monosomic analyses were similar to the results obtained using substitution lines. Both methods show that more chromosomes are involved in the determination of any character and very often critical chromosomes were the same ones. This paper presents the results of monosomic analyses carried out to locate genes for culm length and length of the spike in the hexaploid wheat variety Sava and to compare the results obtained using F1 and F2 monosomic analyses with the results obtained using Sava monosomics. Material and Methods Chinese Spring monosomic lines were crossed to hexaploid wheat cultivar Sava and also backcrossed to Sava five times. Chromosome counts for the Chinese Spring monosomics all F1 plants and Sava monosomics were made in root-tip cells using the Feulgen squash method and also most of the monosomics and F1 plants were checked during meiosis. The F1 plants and the Sava monosomics were grown in the glasshouse but in different years. The F2 populations from F1 monosomics, F2 populations from F1 disomics and parents were grown in field in space sowing 10 x 20 cm. |
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