Single nucleotide polymorphisms (SNPs) of a total 51 strains (standard strains and mutant strains) of D. ananasssae and its relatives maintained in KYORIN-Fly were searched for by partial sequencing of selected 19 loci (11 on X chromosome, and 8 on the 2nd chromosome).

Methods and Results

The sequencing information obtained was compared with the D. ananassae genome sequence on Assembly/Alignment/Annotation of 12 related Drosophila species⁽¹⁾ and SNPs were searched for using novoSNP⁽²⁾.

	chromo. arm	CG
XL (5)	sn	CG32858
	Marf	CG3869
	Actn	CG4376
	zw	CG12529
	v	CG6976
XR (6)	fw	CG1500
	f	CG5424
	Bx	CG6500
	w	CG2759
	N	CG3936
	Yp2	CG2979
2L (8)	CG2604	CG2604
	Su(var)3-7	CG8599
	ca	CG31037
	spn-A	CG7948
	Antp	CG1028
	CG31232	CG31232
	Dl	CG3619
	CG2017	CG2017

References

(1) http://rana.lbl.gov/drosophila/ananassae.html
(2) Weckx, S., Del-Favero, J., Rademaker, R., Claes, L., Cruts, M., De Jonghe, P., Van Broeckhove, C., & De Rijk, P., 2005. novoSNP, a novel computational tool for sequence variation discovery. Genome Research 15: 436-442.