Strain Name: atg9a, atg9b / Allele: jt9

Allele jt9
Strain Name atg9a, atg9b
Type Mutant
Genotype
Affected Gene atg9a, atg9b
Origin and Depositor The University of Tokyo (Noboru Mizushima)
Link to ZFIN
Information
atg9a- and atg9b-double mutants (loss-of-function mutation of both atg9a and atg9b) were generated by CRISPR/Cas9.

10-base deletion was introduced in exon8 of atg9a gene by using CRISPR method.
The heterozygous fish was identified by heteroduplex mobility assay using followed primers:
atg9a-f, CGAGTGGAGTTTGAAGAGCGAGTAC
atg9a-r, GCTCTCGCTTGATCACCTCAGTGTAG
Mutation:
5’-CCGCCTGAGCTCGAGGATCTTGT-3’ Wild-type allele
5’-CCG----------AGGATCTTGT-3’ Mutated allele (-10 bp)

14-base insertion was introduced in exon5 of atg9b gene by using CRISPR method.
The heterozygous fish was identified by heteroduplex mobility assay using followed primers:
atg9b-f, CAGGCAATTCCTGTTTGTGGTCACG
atg9b-r, CATCAGGAAGGGTGACTTTGCTCC
Mutation:
5’-CCAATCGAGCCGTCAATCACACG-3’ Wild-type allele
5’-CCAACATCAATCACACGGGTCAGCCGTCAATCACACG-3’ Mutated allele (+14 bp)
 
The Conditions to Distribute Strains
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Morishita et al. Autophagy is required for maturation of surfactant-containing lamellar bodies in the lung and swim bladder. Cell Rep. 33: 108477(2020)
Prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the recipient shall acquire the consent from (or notify to) the DEPOSITOR for such application.
The BIOLOGICAL RESOURCE shall be used only for basic research purpose (NOT for commercial purpose).
The recipient shall acquire a prior consent from Dr. Noboru Mizushima by E-mail.
Noboru Mizushima（Email: nmizu@m.u-tokyo.ac.jp)  
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Reference

Hideaki Morishita, Yuki Kanda, Takeshi Kaizuka, Haruka Chino, Kazuki Nakao, Yoshimi Miki, Yoshitaka Taketomi, Jun-Lin Guan, Makoto Murakami, Atsu Aiba, Noboru Mizushima Autophagy Is Required for Maturation of Surfactant-Containing Lamellar Bodies in the Lung and Swim Bladder Cell Rep 2020 ; 33(10) : 108477 .

 
Facility RIKEN Center for Brain Science