Strain Name: Tg(EF1a:Smac-GFP11)
|Origin and Depositor||Tokyo Medical and Dental University (Hiroshi Nishina)|
|Link to ZFIN|
In the 40 hpf embryos resulting from crossing the Smac-GFP11 and GFP1-10 Tg lines, bright green fluorescence was observed in some apoptotic cells after UV irradiation.
PCR reaction conditions for genotyping:
[50 μL rxn]
32.5 μL ddH2O
10 μL 5×PrimeSTAR Buffer
4 μL dNTP Mix (2.5 mM each)
1 μL Forward Primer (10 μM) [5'-ATG GCG GCT CTG AGA AGT TGG GTG-3']
1 μL Reverse Primer (10 μM) [5'-GTC GTC GTC CTT GTA GTC AGT GAT CCC-3']
1 μL gDNA
0.5 μL PrimeSTAR HS DNA Polymerase
[Thermal Cycler Conditions]
98℃ – 10 sec – 40 cycles
55℃ – 5 sec – 40 cycles
72℃ – 52 sec – 40 cycles
4℃ - pause
[PCR product: 780-bp]
|The Conditions to Distribute Strains|
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nasu Y, Asaoka Y, Namae M, Nishina H, Yoshimura H1, Ozawa T (2016) Genetically Encoded Fluorescent Probe for Imaging Apoptosis in Vivo with Spontaneous GFP Complementation. Anal Chem, 88:838-844
The BIOLOGICAL RESOURCE shall be used only for basic research purpose (NOT for commercial purpose).
|Facility||RIKEN (Wako) CBS, Lab for Neural Circuit Dynamics of Decision Making|