Strain Name: wnt8a / Allele: ORF1ORF2-1/+

Allele ORF1ORF2-1/+
Strain Name wnt8a
Type Mutant
Affected Gene
Origin and Depositor Nagoya University (Masahiko Hibi)
Link to ZFIN
Deletion mutantions are introduced in both ORF1 and ORF2 of wnt8a gene (-16 bp in ORF1, -9+5 in ORF2) by TALENs. The ORF1 mutation can be detected by PCR with the primers:ORF1-T1-short-F, CAGAGTGGTATAGAAGAGTGCA; ORF1-T1-short-R, CGCTTTCCGGGCAGTTCCACCT. The ORF2 mutation can be detected by PCR with the primers: ORF2-T2-short-F, TGGCATGTCAGAAAGTTGCACA
. Zygotic homozygous mutant embryos show dorsalized and anteriorized phenotypes.  
The Conditions to Distribute Strains
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Facility RIKEN (Wako) CBS, Lab for Neural Circuit Dynamics of Decision Making