Strain Name: Tg(UAS:lifeact-mCherry) / Allele: chi4

Allele chi4
Strain Name Tg(UAS:lifeact-mCherry)
Type Transgenics
Genotype
Affected Gene
Origin and Depositor Chiba University (Motoyuki Ito)
Link to ZFIN ZDB-ALT-161117-2    
Information
This transgenic line can express lifeact fused with mCherry under UAS promotor.Lifeact is F-Actin binding peptide. F-Actin dynamics can be visualized as red fluoresence. To identify this Tg fish, PCR-based genotyping is required using DNA from embryos or adult fins (5'-CATCGCGTCTCAGCCTCAC-3' and 5'-CCCTTCTTCCTTTGAGATGCTTTGG-3').  
The Conditions to Distribute Strains
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
"Zebrafish lines expressing UAS-driven red probes for monitoring cytoskeletal dynamics" Takamasa Mizoguchi, Koichi Kawakami and Motoyuki Itoh. Genesis, 2016  
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Reference

 
Facility RIKEN (Wako) CBS, Lab for Neural Circuit Dynamics of Decision Making