Strain Name: Tg(EF1a:Smac-GFP11) |
Allele | |
Strain Name | Tg(EF1a:Smac-GFP11) |
Type | Transgenics |
Genotype | |
Affected Gene | |
Origin and Depositor | Tokyo Medical and Dental University (Hiroshi Nishina) |
Link to ZFIN |
Information | |
In the 40 hpf embryos resulting from crossing the Smac-GFP11 and GFP1-10 Tg lines, bright green fluorescence was observed in some apoptotic cells after UV irradiation. PCR reaction conditions for genotyping: [50 μL rxn] 32.5 μL ddH2O 10 μL 5×PrimeSTAR Buffer 4 μL dNTP Mix (2.5 mM each) 1 μL Forward Primer (10 μM) [5'-ATG GCG GCT CTG AGA AGT TGG GTG-3'] 1 μL Reverse Primer (10 μM) [5'-GTC GTC GTC CTT GTA GTC AGT GAT CCC-3'] 1 μL gDNA 0.5 μL PrimeSTAR HS DNA Polymerase [Thermal Cycler Conditions] 98℃ – 10 sec – 40 cycles 55℃ – 5 sec – 40 cycles 72℃ – 52 sec – 40 cycles 4℃ - pause [PCR product: 780-bp] |
The Conditions to Distribute Strains | |
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nasu Y, Asaoka Y, Namae M, Nishina H, Yoshimura H1, Ozawa T (2016) Genetically Encoded Fluorescent Probe for Imaging Apoptosis in Vivo with Spontaneous GFP Complementation. Anal Chem, 88:838-844 The BIOLOGICAL RESOURCE shall be used only for basic research purpose (NOT for commercial purpose). |
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Reference | |
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Facility | RIKEN Center for Brain Science |