Strain Name: gsx2 / Allele: ex1-8

Allele ex1-8
Strain Name gsx2
Type Mutant
Genotype
Affected Gene gsx2
Origin and Depositor Nagoya University (Masahiko Hibi)
Link to ZFIN
Information
A deletion mutation (8 bp) was introduced in the exon1of gsx2 gene by a CRISPR/Cas9 method. The mutation can be detected by PCR with the primers:
gsx2-t2-F:5’- CATTGGCATGCACTCTCCCG -3
gsx2-t2-R:5’- TGAGGATACGCACAGCGGAC -3


Mutation:
WT   CAAAAAGTGCCGGTCTTCCGGGACGGGCAGATGGGCGCAGTGACGCTCATCACGCCGG
MUT  CAAAAAGTGCCGGTCTTCCGGGA--------TGGGCGCAGTGACGCTCATCACGCCGG
 
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Reference
 
Facility RIKEN Center for Brain Science