Strain Name: Tg(EF1a:Smac-GFP11)

Allele
Strain Name Tg(EF1a:Smac-GFP11)
Type Transgenics
Genotype
Affected Gene
Origin and Depositor Tokyo Medical and Dental University (Hiroshi Nishina)
Link to ZFIN
Information
In the 40 hpf embryos resulting from crossing the Smac-GFP11 and GFP1-10 Tg lines, bright green fluorescence was observed in some apoptotic cells after UV irradiation.

PCR reaction conditions for genotyping:
[50 μL rxn]
32.5 μL ddH2O
10 μL 5×PrimeSTAR Buffer
4 μL dNTP Mix (2.5 mM each)
1 μL Forward Primer (10 μM) [5'-ATG GCG GCT CTG AGA AGT TGG GTG-3']
1 μL Reverse Primer (10 μM) [5'-GTC GTC GTC CTT GTA GTC AGT GAT CCC-3']
1 μL gDNA
0.5 μL PrimeSTAR HS DNA Polymerase

[Thermal Cycler Conditions]
98℃ – 10 sec – 40 cycles
55℃ – 5 sec – 40 cycles
72℃ – 52 sec – 40 cycles
4℃ - pause
[PCR product: 780-bp]  
The Conditions to Distribute Strains
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nasu Y, Asaoka Y, Namae M, Nishina H, Yoshimura H1, Ozawa T (2016) Genetically Encoded Fluorescent Probe for Imaging Apoptosis in Vivo with Spontaneous GFP Complementation. Anal Chem, 88:838-844
The BIOLOGICAL RESOURCE shall be used only for basic research purpose (NOT for commercial purpose).  
Request To Order
Reference

Nasu Y, Asaoka Y, Namae M, Nishina H, Yoshimura H, Ozawa T. Genetically Encoded Fluorescent Probe for Imaging Apoptosis in Vivo with Spontaneous GFP Complementation. Anal. Chem. 2016 ; 88(1) : 838-844 .

 
Facility RIKEN Center for Brain Science