Strain Name: rgs3a / Allele: -26

Allele -26
Strain Name rgs3a
Type Mutant
Genotype
Affected Gene rgs3a
Origin and Depositor Nagoya University (Masahiko Hibi)
Link to ZFIN
Information
A deletion mutation (26 bp) was introduced in the rgs3a gene by a CRISPR method. The mutation can be detected by PCR with the primers:
rgs3a-26-f: CATCCAATAACATCTCCCCTTC
rgs3a-26-r: CTCTGTGCGCAAGAAAGCTC

Mutation:
WT   CCCCTTCTTCTGCCTCTCTGTTTTTTCTTCCTCTCTCCCTCATCAGATGGACTGGCGGCG
MUT  CCCCTTCTTCTGCCTCTCTTCTGCT--------------------------CTGGCGGCG
-26 bp
 
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Reference
 
Facility RIKEN Center for Brain Science