Strain Name: nrp2b / Allele: ex5+14

Allele ex5+14
Strain Name nrp2b
Type Mutant
Genotype
Affected Gene nrp2b
Origin and Depositor Nagoya University (Masahiko Hibi)
Link to ZFIN
Information
A insertion mutation (+14bp) was introduced in the exon5 of nrp2b gene by a CRISPR method. The mutation can be detected by PCR with the primers:
nrp2b-ex5+14-f: GATTGGACGCCACTGCGGTA
nrp2b-ex5+14-r: TTTGGCCACAGCCATGTCCG

Mutation:
WT   TCCAGTCATCCACTGG--------------AATCCTTTCT
MUT TCCAGTCGTCCACTGGACGATTCCTTTTTCTATCCTTTCT
+14 bp  
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Reference
 
Facility RIKEN Center for Brain Science