Strain Name: ptf1a / Allele: ex1-4

Allele ex1-4
Strain Name ptf1a
Type Mutant
Genotype
Affected Gene ptf1a
Origin and Depositor Nagoya University (Masahiko Hibi)
Link to ZFIN
Information
A deletion mutation (4 bp) was introduced in the exon1 of ptf1a gene by a CRISPR method. The mutation can be detected by PCR with the primers:
ptf1a-ex1-4-f: ACCTCAGAGCTGTCCCCTCACAGA
ptf1a-ex1-4-r: GGCAGCTTGACGCAACTGTT

Mutation:
WT  TCACAGAGACGGAGGCTTACTGAAGAGGCGGAGGCGCATGAGGTCTGAAGTGGAGATGC
MUT TCACAGAGACGGAGGCTTACTGAAGAGGCGGAGGCG----AGGTCTGAAGTGGAGATGC

-4 bp
 
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Reference
 
Facility RIKEN Center for Brain Science