Strain Name: mib2 / Allele: chi1 |
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| Allele | chi1 |
| Strain Name | mib2 |
| Type | Mutant |
| Genotype | |
| Affected Gene | mib2 |
| Origin and Depositor | Chiba University (Motoyuki Ito) |
| Link to ZFIN |
| Information | |
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The Mindbomb E3 ubiquitin protein ligase (Mib) family of proteins, Mib1 and Mib2, are RING finger ubiquitin ligases that share specific substrates. Mib1 is known to play essential roles in Notch signalling by ubiquitinating Notch ligands in vivo. Conversely, the functions of Mib2 in vivo are not fully understood. To determine functions of Mib2 in vivo, we generated mutant alleles of zebrafish mib2 using transcription activator-like effector nucleases (TALENs). We found that mib2 homozygous mutants were viable and fertile. Notch-mediated functions, such as early neurogenesis, somitogenesis, and pigment cell development, were not affected in mib2 mutant embryos. mib2/chi1, feature 8 bp deletions. The mutation created stop codons in the first ankyrin repeat domain (Mib2chi1, A538stop). |
| The Conditions to Distribute Strains | |
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In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mikami et al., Biol Open. 2015 Oct 30;4(11):1576-82. |
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| Facility | RIKEN Center for Brain Science |