Strain Name: pbxip1b / Allele: ex9-14

Allele ex9-14
Strain Name pbxip1b
Type Mutant
Genotype
Affected Gene pbxip1b (ENSDARG00000011824)
Origin and Depositor Nagoya University (Masahiko Hibi)
Link to ZFIN
Information
a 14-bp deletion (30-bp deletion and 16-bp insertion) was introduced in the exon9 of pbxip1b gene by CRISPR/Cas9 method. The mutation can be detected by PCR with the primers:
pbxip1b-t3-F: 5'-CAAAATGGTCTTTTCTGTCCTC-3'
pbxip1b-t3-R: 5'-TTCCTGTTCCTTATGTCTGGTC-3'

Mutation:
WT    CTCCAGCAGGGGGGGTCCGAGAGGTGGCAATGGGATGCTGATGTTCTGCTTTTACT
MUT   CTCCAGCAGGG--------------ACTAATCCCATCCCATTGTTCTGCTTTTACT
The blue and red letters indicate deleted and inserted nucleotides, respectively.  
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Reference
 
Facility RIKEN Center for Brain Science