Strain Name: ky1-AB

Allele
Strain Name ky1-AB
Type Transgenics
Genotype
Affected Gene
Origin and Depositor National Institute for Environmental Studies (Yasunobu Aoki)
Link to ZFIN ZDB-ALT-090105-1    
Information
This transgenic zebrafish line carries a shuttle vector plasmid (pML4) for detecting mutagens in aquatic environments. This plasmid contains the rpsL gene of Escherichia coli as a mutational target gene, and the kanamycin-resistance gene for recovering the plasmid from the chromosomal DNA. About 350 copies of pML4 plasmid per haploid genome were integrated into a single site in a tandem array on the chromosome. This transgenic line established from AB line is phenotypically identical to the wild type, and has been maintained as a hemizygote. A transgenic fish has been selected by dot blot analysis, and the protocol was provided from the developer.  
The Conditions to Distribute Strains
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Amanuma, K., Takeda, H., Amanuma, H., and Aoki, Y. Transgenic zebrafish for detecting mutations caused by compounds in aquatic environments. Nat. Biotechnol. (2000) 18(1): 62-65
Prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the recipient shall acquire the consent from (or notify to) the DEPOSITOR for such application.
Prior to publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the recipient shall acquire the consent from the DEPOSITOR.
The recipient and the DEPOSITOR shall sign an MTA, which set forth the terms and conditions to use the intellectual property rights based on the results of research using the BIOLOGICAL RESOURCE and so on. The recipient shall be sent an agreement for distribution obtained from the DEPOSITOR to NZC.  
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Reference

Amanuma K, Takeda H, Amanuma H, Aoki Y. Transgenic zebrafish for detecting mutations caused by compounds in aquatic environments. Nat. Biotechnol. 2000 ; 18(1) : 62-65 .

Amanuma K, Tone S, Saito H, Shigeoka T, Aoki Y. Mutational spectra of benzo[a]pyrene and MeIQx in rpsL transgenic zebrafish embryos. Mutat. Res. 2002 ; 513(1-2) : 83-92 .

 
Facility RIKEN Center for Brain Science