Strain Name: CH15H11orf87 / Allele: ex2-11

Allele ex2-11
Strain Name CH15H11orf87
Type Mutant
Genotype
Affected Gene CH15H11orf87
Origin and Depositor Nagoya University (Masahiko Hibi)
Link to ZFIN
Information
a 11-bp deletion was introduced in the exon2 of C15H11orf87 gene by CRISPR/Cas9 method. The mutation can be detected by PCR with the primers:
C15H11orf87-t6-F: 5'-CCCCCTGCAATACAGACACT-3'
C15H11orf87-t6-R: 5'-GGTTCCGTTTAAGGCACAGT-3'

Mutation:
WT    ATGAGTGCCCGGACCTTGGAGAGACTGGCGCTTTCCATTC
MUT   ATGAGTGCCCGGAC-----------TGGCGCTTTCCATTC 
The blue letters indicate deleted nucleotides.  
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Reference
 
Facility RIKEN Center for Brain Science