Protocols

Protocol for Raising Paramecia ( PDF download )

In any questions, contact: Satomi Nakayama

Baby fish that are one to two weeks old are fed paramecia.

This protocol describes how to raise paramecia and how to feed baby fish with paramecia.

Protocol for for freezing zebrafish sperm and in vitro fertilization ( PDF download )

In any questions, contact: Satomi Nakayama

This protocol describes how to freeze zebrafish sperm and do in vitro fertilization.

Protocol for in vitro storage of zebrafish and medaka sperm at room temperature（ PDF download ）

In any questions, contact: Noriyoshi Sakai

This manuscript describes how to store zebrafish and medaka sperm in vitro at room temperature.

Call for protocols

Your protocols related to zebrafish experiments are welcome. Any information on zebrafish breeding, maintenance, etc, which may be useful to zebrafish researchers, is also welcome. Please cooperate with us for further improvement of zebrafish research.

Submitting protocols

If you are going to submit protocols or other information, please contact Akiko Ishioka by e-mail (Akiko Ishioka).
Please attach a word file of the protocol or other information. Please also attach a file of a short outline of the document. Please specify the title of the protocol, authors, institution, and contact information.

Confidentiality

We are planning to make restrictions to download the protocols, such that the users have to agree the conditions that the authors specified. Please let us know if you can make the information open to the public without any restriction. If you are going to make a restriction, please let us know the condition.

For example:

The uses have to register and identify themselves.
In addition to the registration, the users have to acknowledge when they publish the experimental results using the protocol.
The users have to cite the papers listed by the authors, when they publish the experimental results using the protocol.