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Author Causse, M.A., T.M. Fulton, Y.G. Cho, S.N. Ahn, J. Chunwongse, K. Wu, J. Xiao, Z. Yu, P.C. Ronald, S.E. Harrington, G. Second, S.R. McCouch and S.D. Tanksley
Title Saturated molecular map of the rice genome based on an interspecific backcross population.
Abstract:
A molecular map has been constructed for the rice genome comprised of 726 markers (mainly restriction fragment length polymorphisms; RFLPs). The mapping population was derived from a backcross between cultivated rice, Oryza sativa, and its wild African relative, Oryza longistaminata. The very high level of polymorphism between these species, combined with the use of polymerase chain reaction-amplified cDNA libraries, contributed to mapping efficiency. A subset of the probes used in this study was previously used to construct an RFLP map derived from an inter subspecific cross, providing a basis for comparison of the two maps and of the relative mapping efficiencies in the two crosses. In addition to the previously described PstI genomic rice library, three cDNA libraries from rice (Oryza), oat (Avena) and barley (Hordeum) were used in this mapping project. Levels of polymorphism detected by each and the frequency of identifying heterologous sequences for use in rice mapping are discussed. Though strong reproductive barriers isolate O. sativa from O. longistaminata, the percentage of markers showing distorted segregation in this backcross population was not significantly different than that observed in an intraspecific F2 population previously used for mapping. The map contains 1491 cM with an average interval size of 4.0 cM on the framework map, and 2.0 cM overall. A total of 238 markers from the previously described PstI genomic rice library, 250 markers from a cDNA library of rice (Oryza), 112 cDNA markers from oat (Avena), and 20 cDNA markers from a barley (Hordeum) library, two genomic clones from maize (Zea), 11 microsatellite markers, three telomere markers, eleven isozymes, 26 cloned genes, six RAPD, and 47 mutant phenotypes were used in this mapping project. Applications of a molecular map for plant improvement are discussed.
Journal Genetics
Country
Volume 138
Pages 1251-1274.
Year 1994
PubMed ID 7896104
PubMed Central ID -
DOI -
URL -
Relation
Gene ACP1 ACP2 ACP4 ACS1 ACS3 ACT1 ADH1 AMY1A AMY1B AMY1C AMY2A AMY3A AMY3B AMY3C AMY3D AMY3E ATP ATUB BHC BPH10 CALA CALB CHT1 CL CYC1 D27 D5 DN1 EF EST EST2 GL1 GLH GM2 HBV IPI IPI3 LA LAX1 LEC LRT2 MAL1 PGD1 PGI2 PHYA PI PI10 PI11 PI157 PI30 PI5 PI6 PI9 PITA PIZ PIZH POX2 PR R45S1 R5S1 RCH10 S5 SALT SD1 SDH1 SE3 SK2(T)(SCL, FGR) TELSA1 TELSM1 TELSM3 TRYP TSV1 WBPH1 WX1 XA1 XA10 XA2 XA21 XA4 XA5 Z2 _
INSD -
Strain Wild Core Collection -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
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Stages in Each Organ
- Muant Lines (Gene)
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Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase