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Reference Detail
| Reference | ||
|---|---|---|
| Author | Wang Z., Zhang J., Huang J., Sha G., Song X., Cao X., Yan Z., Liu C., Chen S., Li Z., Huang X., Xie Q., Yang X., Zhou G., Zhang T. | |
| Title | Plant negative-strand RNA virus phosphoprotein condensates exploit host trafficking and lipid synthesis for viral factory assembly. | |
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Abstract: RNA viruses often remodel host intracellular membranes to establish specialized replication compartments through viral protein-induced phase separation. However, the mechanisms underlying membrane remodeling and the characteristics that render these sites conducive to replication remain poorly understood, particularly in plant negative-strand RNA viruses. Here, we demonstrate that the phosphoprotein (P) of rice stripe mosaic virus (RSMV) forms biomolecular condensates via liquid-liquid phase separation (LLPS) to recruit essential components for viral replication factories (VFs). We identify a direct interaction between RSMV P and adenosine diphosphate (ADP) ribosylation factor 1 (OsARF1C), a crucial regulator of the coatomer protein I (COP I) vesicle transport pathway that is vital for viral replication. This interaction indirectly recruits OsARF1C's partner, phosphatidylinositol 4-kinase beta (OsPI4KB), which drives localized phosphatidylinositol-4 phosphate (PI4P) synthesis. Concurrently, the P protein modulates its aggregates and LLPS droplets through PI4P, thereby expanding the replication site and enhancing viral replication. |
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| Journal | Sci Adv | |
| Country | China | |
| Volume | 11(34) | |
| Pages | eadx7905 | |
| Year | 2025 | |
| PubMed ID | 40834074 | |
| PubMed Central ID | 12366689 | |
| DOI | 10.1126/sciadv.adx7905 | |
| URL | - | |
| Relation | ||
| Gene | ARF2B ARFA1A ARFA1B ARFA1C ARFA1D ARFA1E PI4K1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
