Update info.Tools - Reference - Detail
Reference Detail
| Reference | ||
|---|---|---|
| Author | Liu L., Hao J., Huang K., Duan P., Zhang B., Chi Z., Yao X., Li Y. | |
| Title | Redox regulation of G protein oligomerization and signaling by the glutaredoxin WG1 controls grain size in rice. | |
|
Abstract: grain size is an important agronomic trait and influences both grain yield and quality in crops. The atypical heterotrimeric Gγ protein subunit GS3 is a central regulator of grain length in rice, and the loss-of-function allele of its corresponding gene has been widely utilized by breeders to improve grain length in rice. Here we report that the CC-type glutaredoxin WG1/OsGRX8 has disulfide oxidoreductase activity and regulates redox state of GS3, thereby determining grain length in rice. GS3 can form dimers and oligomers by intermolecular disulfide bonds, and the cysteine-rich C-terminal region of GS3 is predominantly required for its oligomerization. The oligomerization of GS3 alleviates its inhibitory effect on the interaction between RGB1 and DEP1/GGC2, resulting in an increase in grain length. WG1 interacts with GS3 and reduces the oligomerization of GS3 through redox mechanisms, which causes a decrease in grain length. Genetic analyses support WG1 and GS3 function in a common pathway to control grain length. Thus, our findings reveal a previously unrecognized mechanism, in which redox regulation of a Gγ subunit by a glutaredoxin controls grain length, opening a novel perspective for G protein signaling regulation. |
||
| Journal | EMBO J. | |
| Country | China | |
| Volume | 44(13) | |
| Pages | 3742-3763 | |
| Year | 2025 | |
| PubMed ID | 40389777 | |
| PubMed Central ID | 12216599 | |
| DOI | 10.1038/s44318-025-00462-9 | |
| URL | - | |
| Relation | ||
| Gene | DEP1 GGC2 LK3 RGB1 WG1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
