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Reference Detail
| Reference | ||
|---|---|---|
| Author | Jung Y.J., Go J., Kim J.Y., Lee H.J., Kim J.H., Lee H.M., Cho Y.G., Kang K.K. | |
| Title | ABA and Pre-Harvest Sprouting Differences in Knockout Lines of OsPHS3 Encoding Carotenoid Isomerase via CRISPR/Cas9 in Rice. | |
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Abstract: We generated and characterized knockout mutant lines of the <i>OsPHS3</i> gene using the CRISPR/Cas9 system. The knockout lines of the <i>OsPHS3</i> gene showed that 1 bp and 7 bp deletion, early termination codons were used for protein production. Agronomic characteristics of knock-out lines were reduced in plant height, culm diameter, panicle length, seed size and weight, except for the number of tillers. In addition, we analyzed the expression levels of carotenoid biosynthesis genes by qRT-PCR. Among the genes encoding carotenoid metabolic pathway enzymes, the level of transcripts of <i>PSY1</i>, <i>PSY2</i>, <i>PSY3</i>, <i>PDS</i> and <i>ZDS</i> were higher in the KO lines than in the WT line. In contrast, transcription of the ε<i>-LCY</i>, <i>β</i>-<i>LCY</i> and <i>ZEP1</i> genes were downregulated in the KO lines compared to the WT line. Also, the KO lines decreased carotenoid content and ABA amount compared to WT, while preharvest sprouts increased. These results suggested that they would certainly help explain the molecular mechanisms of PHS in other crops, such as wheat and barley, which are susceptible to PHS. |
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| Journal | Plants (Basel) | |
| Country | South Korea | |
| Volume | 14(3) | |
| Pages | ||
| Year | 2025 | |
| PubMed ID | 39942907 | |
| PubMed Central ID | 11820545 | |
| DOI | 10.3390/plants14030345 | |
| URL | - | |
| Relation | ||
| Gene | LCYB LYCE PDA PHS3 PSY1 PSY2 PSY3 ZDS ZEP1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
