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Reference Detail
| Reference | ||
|---|---|---|
| Author | Chen D.B., Zhou R., Wang H.M., Zhang P.P., Yang Z.F., Xuan D.D., Zhang Y.X., Zhan X.D., Cao L.Y., Cheng S.H., Sun L.P. | |
| Title | <i>OsLAP3/OsSTRL2</i>, encoding a rice strictosidine synthase, is required for anther cuticle formation and pollen exine patterning in rice. | |
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Abstract: The formation of the anther wall and the development of pollen processes, central to rice fertility and yield, are highly dependent on the synthesis and accumulation of lipid polymers. Although several regulatory factors related to lipid biosynthesis during pollen wall development have been identified, the molecular mechanisms controlling these processes remain poorly understood. In this study, a male-sterile rice mutant, <i>lap3</i>, was identified, characterized by normal vegetative growth but complete male sterility due to delayed programmed cell death (PCD) in tapetal cells and defects in anther cuticle and pollen exine formation. Map-based cloning revealed that <i>OsLAP3</i> is a new allele of the strictosidine synthase-like gene, <i>OsSTRL2</i>. Functional analysis, including complementation and CRISPR/Cas9-based gene editing, confirmed that the 2-nucleotide deletion in the <i>OsLAP3</i> is responsible for the male sterility phenotype. <i>OsLAP3</i> is homologous to the maize <i>ZmMS45</i>, the core recessive nuclear sterile gene of maize Seed Production Technology (SPT), and localizes to the endoplasmic reticulum and plays a conserved role in anther development and pollenformation. Gene expression analysis revealed a significant downregulation of key genes involved in anther development and sporopollenin biosynthesis in <i>lap3</i> anthers. Furthermore, lipid profiling demonstrated a marked reduction in both wax and cutin content. These findings establish <i>OsLAP3</i> as a critical regulator of fatty acid synthesis and highlight its role in anther cuticle formation and pollen exine development. The findings of this study provide valuable insights into the molecular regulation of lipid biosynthesis during rice male reproductive development and offer potential applications for hybrid rice breeding. |
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| Journal | Front Plant Sci | |
| Country | China | |
| Volume | 15 | |
| Pages | 1508828 | |
| Year | 2024 | |
| PubMed ID | 39902213 | |
| PubMed Central ID | 11789761 | |
| DOI | 10.3389/fpls.2024.1508828 | |
| URL | - | |
| Relation | ||
| Gene | CYP703A3 CYP704B2 EAT1 LAP3 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
