Update info.Tools - Reference - Detail
Reference Detail
| Reference | ||
|---|---|---|
| Author | Zhou S., Zhang R., Wang Q., Zhu J., Zhou J., Sun Y., Shen S., Luo J. | |
| Title | OsbHLH5 Synergically Regulates Phenolamide and Diterpenoid Phytoalexins Involved in the Defense of Rice Against Pathogens. | |
|
Abstract: Rice (<i>Oryza sativa</i>) produces phenolamides and diterpenoids as major phytoalexins. Although the biosynthetic pathways of phenolamides and diterpenoids in plants have been revealed, knowledge of their accumulation regulatory mechanisms remains limited, and, in particular, no co-regulatory factor has been identified to date. Here, using a combined co-expression and evolutionary analysis, we identified the basic helix-loop-helix (bHLH) transcription factor <i>OsbHLH5</i> as a positive bifunctional regulator of phenolamide and diterpenoid biosynthesis in rice. Metabolomic analysis revealed that <i>OsbHLH5</i> significantly increased the content of phenolamides (such as feruloyl tryptamine (Fer-Trm) and <i>p</i>-coumaroyl tyramine (Cou-Tyr)) and diterpenoid phytoalexins (such as momilactones A, momilactones B) in the overexpression lines, while their content was reduced in the <i>OsbHLH5</i> knockout lines. Gene expression and dual-luciferase assays revealed that <i>OsbHLH5</i> activates phenolamide biosynthetic genes (including <i>putrescine hydroxycinnamoyltransferase 3</i> (<i>OsPHT3</i>), <i>tyramine hydroxycinnamoyltransferases 1/2</i> (<i>OsTHT1/2</i>), and <i>tryptamine benzoyltransferase 2</i> (<i>OsTBT2</i>)) as well as diterpenoid biosynthetic genes (including <i>copalyl diphosphate synthase 4</i> (<i>OsCPS4</i>) and <i>kaurene synthase-like 4/7/10/11</i> (<i>OsKSL4/7/10/11</i>)). Furthermore, we have demonstrated that <i>OsbHLH5</i> is induced by jasmonic acid (JA), while pathogen inoculation assays indicated that the overexpression of <i>OsbHLH5</i> in transgenic rice plants leads to enhanced resistance to <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>). Overall, we have identified a positive regulator of phenolamide and diterpenoid biosynthesis and have demonstrated that biotic stress induces phytoalexin accumulation partly in an <i>OsbHLH5</i>-dependent manner, providing new insights into the metabolic interactions involved in pathogen response and offering valuable gene resources for the development, through genetic improvement, of new rice varieties that are resistant to diseases. |
||
| Journal | Int J Mol Sci | |
| Country | China | |
| Volume | 25(22) | |
| Pages | ||
| Year | 2024 | |
| PubMed ID | 39596224 | |
| PubMed Central ID | 11595221 | |
| DOI | 10.3390/ijms252212152 | |
| URL | - | |
| Relation | ||
| Gene | BHLH27 CPS4 KS4 KS7 KS8 KSL10 PHT3 TBT2 THT1 THT2 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
