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Reference Detail
| Reference | ||
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| Author | Yan B., Liu C., Sun J., Mao Y., Zhou C., Li J., Liu W., Li S., Yan W., Fu C., Qin P., Fu X., Zhao X., Song X., Nie J., Gao F., Yang Y., Chen Y., Cao X. | |
| Title | Impaired 2',3'-cyclic phosphate tRNA repair causes thermo-sensitive genic male sterility in rice. | |
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Abstract: Hybrid rice, widely planted in Asia, is pathogen resistant and has superior yields, making it a major contributor to global food security. The two-line hybrid rice system, which utilizes mutants exhibiting photo-/thermo-sensitive genic male sterility (P/TGMS), is the leading hybrid rice breeding technology. Mutations in THERMO-SENSITIVE GENIC MALE STERILE 5 (tms5) accounts for over 95% of current TGMS lines. We previously found that tms5 carries a mutation in ribonuclease ZS1. Despite its importance for breeding robust rice lines, the mechanism underlying tms5-mediated TGMS remains elusive. Here, we demonstrate that tms5 is a tRNA 2',3'-cyclic phosphatase. The tms5 mutation leads to accumulation of 2',3'-cyclic phosphate (cP)-ΔCCA-tRNAs (tRNAs without 3' CCA ended with cP), which is exacerbated by high temperatures, and reduction in the abundance of mature tRNAs, particularly alanine tRNAs (tRNA-Alas). Overexpression of tRNA-Alas in the tms5 mutant restores male fertility to 70%. Remarkably, male fertility of tms5 mutant is completely restored at high temperatures by knocking out OsVms1 which encodes the enzyme for cP-ΔCCA-tRNA generation. Our study reveals the mechanism underlying tms5-mediated TGMS in rice and provides mechanistic insight into the further improvement of TGMS in hybrid crop development. |
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| Journal | Cell Res. | |
| Country | China | |
| Volume | 34(11) | |
| Pages | 763-775 | |
| Year | 2024 | |
| PubMed ID | 39251844 | |
| PubMed Central ID | 11528004 | |
| DOI | 10.1038/s41422-024-01012-4 | |
| URL | - | |
| Relation | ||
| Gene | TMS5 ZOS4-12 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
