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Reference Detail
| Reference | ||
|---|---|---|
| Author | Feng Z., Xu Y., Xie Z., Yang Y., Lu G., Jin Y., Wang M., Liu M., Yang H., Li W., Liang Z. | |
| Title | Overexpression of Abscisic Acid Biosynthesis Gene <i>OsNCED3</i> Enhances Survival Rate and Tolerance to Alkaline Stress in Rice Seedlings. | |
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Abstract: Alkaline stress with high pH levels could significantly influence plant growth and survival. The enzyme 9-cis-epoxycarotenoid dioxygenase (NCED) serves as a critical bottleneck in the biosynthesis of abscisic acid (ABA), making it essential for regulating stress tolerance. Here, we show that <i>OsNCED3</i>-overexpressing rice lines have increased ABA content by up to 50.90% and improved transcription levels of numerous genes involved in stress responses that significantly enhance seedling survival rates. Overexpression of <i>OsNCED3</i> increased the dry weight contents of the total chlorophyll, proline, soluble sugar, starch, and the activities of antioxidant enzymes of rice seedlings, while reducing the contents of O2·-, H2O2, and malondialdehyde under hydroponic alkaline stress conditions simulated by 10, 15, and 20 mmol L-1 of Na2CO3. Additionally, the <i>OsNCED3</i>-overexpressing rice lines exhibited a notable increase in the expression of <i>OsNCED3</i>; ABA response-related genes <i>OsSalT</i> and <i>OsWsi18</i>; ion homeostasis-related genes <i>OsAKT1</i>, <i>OsHKT1;5</i>, <i>OsSOS1</i>, and <i>OsNHX5</i>; and ROS scavenging-related genes <i>OsCu/Zn-SOD</i>, <i>OsFe-SOD</i>, <i>OsPOX1</i>, <i>OsCATA</i>, <i>OsCATB</i>, and <i>OsAPX1</i> in rice seedling leaves. The results of these findings suggest that overexpression of <i>OsNCED3</i> upregulates endogenous ABA levels and the expression of stress response genes, which represents an innovative molecular approach for enhancing the alkaline tolerance of rice seedlings. |
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| Journal | Plants (Basel) | |
| Country | China | |
| Volume | 13(12) | |
| Pages | ||
| Year | 2024 | |
| PubMed ID | 38931145 | |
| PubMed Central ID | 11207436 | |
| DOI | 10.3390/plants13121713 | |
| URL | - | |
| Relation | ||
| Gene | AKT1 APX1 ATP6 CATA CATB GPA6 NCED1 SALT SKC1 SOD4 SODB SOS1 WSI18 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
